- Title
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Environmental microplastics disrupt swimming activity in acute exposure in Danio rerio larvae and reduce growth and reproduction success in chronic exposure in Danio rerio and Oryzias melastigma
- Authors
- Cormier, B., Cachot, J., Blanc, M., Cabar, M., Clérandeau, C., Dubocq, F., Le Bihanic, F., Morin, B., Zapata, S., Bégout, M.L., Cousin, X.
- Source
- Full text @ Environ. Pollut.
Fig. 1. Body length and weight of zebrafish (A and B) or marine medaka (C and D) at 6 mpf, after MG and PB MPs exposures. (Mean ± SD, Tukey HSD test, * indicates significant differences from respective control of same sex with * p < 0.05; **p < 0.001; ***p < 0.0001; n = 11 to 53 per sex and per treatment). |
Fig. 2. Relative proportions of successful (white) and failed (black) attempts to obtain eggs from zebrafish pairs exposed to MG or PB MPs. (Fisher's exact test; ***: p < 0.0001. Attempts n = 56–66 from three replicates). |
Fig. 3. Reproductive output of marine medaka exposed to MG or BP MPs. The number of eggs per day normalised according to the number of females present in the tank. R square of linear regressions are all above 0.9 (***: p < 0.0001; Attempts n = 18–38 from three replicates). |
Fig. 4. Individual standard length of F1 larvae of adults zebrafish (left) and marine medaka (right) exposed to MG or PB MPs relative to control. (Mean ± SD; ANOVA; *: p < 0.05; **p < 0.01; zebrafish n = 9–15; marine medaka n = 44–60). |
Fig. 5. Larval photomotor response in F1 generation, (A) 5 dpf zebrafish and (B) 14 dpf marine medaka after parental exposure to MG and PB MPs. Distance travelled over 5 min periods including two light-on periods (LON1, LON2) and one light off period (LOFF). (Mean ± SD repeated–measure ANOVA; differences between treatments within the same observation period as *p < 0.05; ***p < 0.001; n = 50 to 60 larvae per treatment). |