- Title
-
A suitable anaesthetic protocol for metamorphic zebrafish
- Authors
- Owen, J.P., Kelsh, R.N.
- Source
- Full text @ PLoS One
SL is the distance from the snout to the caudal peduncle. In pre-flexion larvae that do not have a caudal penduncle, SL is the distance from the snout to the posterior tip of the notochord. |
(A), (C), (E) Percentage of fish that recovered by length for concentrations of 0.008%, 0.009% and 0.01% MS-222 respectively. (B), (D), (F) Percentage of fish that were not MUA for concentrations of 0.008%, 0.009% and 0.01% MS-222 respectively. Black lines represent the 90% confidence interval for each value, calculated using the sample size (see |
Boxplots of the time taken to (A) induce anaesthesia, (B) lose touch responsiveness, (C) regain movement post anaesthesia and (D) regain equalibrium post anaesthesia for each protocol 1–6 as defined in |
Barchart with 95% confidence intervals for age vs length (SL (mm)) of fish anaesthetised using protocols 1,4,5 and 6 respectively. |
Barplot of the mean (A) induction time, (B) time taken to lose touch responsivity, (C) heartbeats per minute at 5:00, (D) visible breaths per minute at 5:00, (E) time taken to regain movement and (F) time taken to fully recover against repeat number for each protocol 1,4,5 and 6. Mean taken over all repeats. P/L corresponds to Propofol/Lidocaine, PE corresponds to phenoxyethanol, CP corresponds to Cotton Pad maintenance. |
Boxplot of (A) induction time, (B) time taken to lose touch responsiveness, (C) time taken to regain movement and (D) time taken to recover. Stars indicate significant difference of subsequent repeats as determined using the Mann-Whitney U test. ns = not significant, ‘*’ indicates p<0.05, ‘**’ indicates p<0.01, ‘***’ indicates p < 0.001 and ‘****’ indicates p<0.0001. |