- Title
-
Protective Role of Galanin during Chemically Induced Inflammation in Zebrafish Larvae
- Authors
- Nowik, N., Przyborowska, A., Sienkiewicz, W., Podlasz, P.
- Source
- Full text @ Biology (Basel)
Galanin expression before (CTRL) and after copper sulfate treatment (CuSO4). Galanin is approximately 4 times upregulated after copper sulfate exposure compared to the untreated group. Data and mean ± SEM were pulled from three independent experiments. **** p < 0.0001 (determined using unpaired t test). |
Cell degeneration after copper sulfate treatment. Number of fluorescent pixels after copper sulfate exposure in the wild-type galn+/+ group (galn+/+), a line with galanin knockout galn−/− (galn−/−) and wild-type galn+/+ treated with galanin analog NAX 5055 (NAX) and a line with galanin overexpression Tg(hsp70l:galn) (galn-HS). Data and mean ± SEM were pulled from three independent experiments. ** p < 0.01; *** p < 0.001 (determined using ANOVA with Tukey’s post hoc test). |
Quantitative PCR of irg1l, il1b and tnfa after copper sulfate incubation. (A–C) qPCR of irg1l, il1b and tnfa after incubation with copper sulfate in galn+/+, galn−/− and wild-type galn+/+ treated with galanin analog NAX 5055 (NAX) and a line with galanin overexpression Tg(hsp70l:galn) (galn-HS) groups was performed at 3 dpf. Control wild-type galn+/+ larvae (galn+/+ ctrl) were incubated only in PBS without copper sulfate. Data and mean ± SEM were pulled from three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001 (determined using ANOVA with Tukey’s post hoc test); ns, not significant. |