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EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Days 45-89

(A) Myc-tagged zebrafish LZTFL1 plasmids were transfected into HeLa cells. At 48 h post-transfection, cells were lysed for Western blot analysis. As shown in the picture, compared with empty vectors, LZTFL1 fused with Myc tag was ectopically expressed in HeLa cells. (B) Myc-tagged zebrafish LZTFL1 was transfected into HeLa cells. At 48 h post-transfection, cells were fixed and probed with FITC-anti-Myc antibodies and DAPI (nuclear counterstain). Moreover, zebrafish LZTFL1 showed predominant localization in the cytoplasm but subtle localization in nuclei. Magnification, 200×. EV, empty vector, pcDNA6-Myc; ZeLZ, pcDNA6-Myc-ZeLZTFL1, zebrafish LZTFL1 was inserted into pcDNA6-Myc.

 

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.