- Title
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Molecular Characterization and Functional Analysis of Leucine Zipper Transcription Factor Like 1 in Zebrafish (Danio rerio)
- Authors
- Wei, Q., Chen, Y., Gu, Y.F., Zhao, W.
- Source
- Full text @ Front. Physiol.
ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. EXPRESSION / LABELING:
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(A) Myc-tagged zebrafish LZTFL1 plasmids were transfected into HeLa cells. At 48 h post-transfection, cells were lysed for Western blot analysis. As shown in the picture, compared with empty vectors, LZTFL1 fused with Myc tag was ectopically expressed in HeLa cells. (B) Myc-tagged zebrafish LZTFL1 was transfected into HeLa cells. At 48 h post-transfection, cells were fixed and probed with FITC-anti-Myc antibodies and DAPI (nuclear counterstain). Moreover, zebrafish LZTFL1 showed predominant localization in the cytoplasm but subtle localization in nuclei. Magnification, 200×. EV, empty vector, pcDNA6-Myc; ZeLZ, pcDNA6-Myc-ZeLZTFL1, zebrafish LZTFL1 was inserted into pcDNA6-Myc.
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