- Title
-
Chemical reprogramming enhances homology-directed genome editing in zebrafish embryos
- Authors
- Aksoy, Y.A., Nguyen, D.T., Chow, S., Chung, R.S., Guillemin, G.J., Cole, N.J., Hesselson, D.
- Source
- Full text @ Commun Biol
Overview of the in vivo homology-directed repair (HDR) detection system. |
Chemical enhancement of CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9-mediated homology-directed repair (HDR) efficiency. Effects of |
Single fiber analysis of homology-directed repair (HDR) events. Cross-sectional in vivo imaging of a zebrafish embryo (3 dpf) co-injected with NU7441 at 50 µM and RS-1 at 30 µM. |
To test if any random donor (tdTomato) insertion is seen when the donor is co-injected with a known Crispr (targeting golden locus) that is not specific to the eBFP2 target site, zebrafish embryos were co-injected with the following material: 1) (A) WT: Tomato 2) (B) WT: Golden Crispr/Ca9 + Tomato 3) (C) WT: Golden Crispr/Ca9 + Tomato + Drugs Multi-channel analysis of bright-field and red-fluorescence showed no red fibers in any of the experimental groups. Scale bars: 750 µm, main image and 250 µm, inset. |