- Title
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A Notch-mediated, temporal asymmetry in BMP pathway activation promotes photoreceptor subtype diversification
- Authors
- Cau, E., Ronsin, B., Bessière, L., Blader, P.
- Source
- Full text @ PLoS Biol.
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(A-B) Representative confocal sections showing the expression of the Tg(aanat2:gfp)y8(aanat2:gfp) transgene in Tg(hsp70l:dnXla.Rbpj-MYC)vu21 (referred to as Notch KD) and wt siblings at 42 hpf. (C-F) Expression of exorh and PT in Tg(hsp70l:dnXla.Rbpj-MYC)vu21 and siblings at 48 hpf (C-D) and 66 hpf (E-F). (G) Counts of Tg(aanat2:gfp)y8+ cells at various stages in Tg(hsp70l:dnXla.Rbpj-MYC)vu21 and siblings. The stage is indicated on the x-axis. (H) Counts of exorh+ and PT+ cells in Tg(hsp70l:dnXla.Rbpj-MYC)vu21 and sibling embryos after in situ hybridization. Stages are 48 hpf for exorh+ and 66 hpf for PT+ cells. Scale bars represents 10 μm. Heat shock was performed at 14 hpf. Error bars represent SD. *p < 0.05, **p < 0.001 using a Mann Whitney test. Underlying data can be found in S1 Data. exorh, exorhodopsin; hpf, hours post fertilization; KD, knock-down; PhR, photoreceptor; PT, parietopsin; wt, wild type. |
(A) Scheme of the experimental design showing the timing of the heat shocks and analysis of the number of exorh+ and PT+ cells. (B-G) In situ hybridization for exorh and PT in 48-hpf WT and Tg(hsp70:bmp2b)fr13 transgenic embryos (BMP GOF) heat shocked at 10 or 16 hpf. Embryos are viewed dorsally with anterior to the top. Scale bar represents 10 μm. (H-I) Counts of exorh+ and PT+ cells in WT and Tg(hsp70:bmp2b)fr13embryos after a heat shock performed at various stages (indicated on the x-axis). Error bars represent SD. *p < 0.05. **p < 0.001. ***p < 0.0005. ****using a Kruskal-Wallis test with Dunn’s post hoc comparisons of the transgenic versus WT populations. Tg(hsp70:bmp2b)fr13 transgenic embryos were labeled BMP GOF. Underlying data can be found in S1 Data. BMP, bone morphogenetic protein; exorh, exorhodopsin; GOF, gain of function; hpf, hours post fertilization; PhR, photoreceptor; PT, parietopsin; WT, wild type. |
Alteration of Notch activity modifies the expression of the Tg(BMPRE-AAV.Mlp:d2EGFP)mw30transgene. (A-B) Confocal projections of WT and Tg(hsp70l:dnXla.Rbpj-MYC)vu21 (Notch KD) embryos at 22 hpf. Embryos are shown in dorsal views. The Tg(BMPRE-AAV.Mlp:d2EGFP)mw30 transgene (BRE:d2EGFP) is shown in green. Scale bar is 15 μm. (C) Counts of dGFP+ cells in WT and Tg(hsp70l:dnXla.Rbpj-MYC)vu21 (Notch KD) embryos at 23 hpf. Underlying data can be found in S2 Data. Heat shock was performed at 14 hpf. Error bars represent SD. *p < 0.05 using a t test. Underlying data can be found in S2 Data. D2EGFP, destabilized enhanced green fluorescent protein; KD, knock-down; hpf, hours post fertilization; WT, wild-type. |