- Title
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FGF2 Induces Migration of Human Bone Marrow Stromal Cells by Increasing Core Fucosylations on N-Glycans of Integrins
- Authors
- Awan, B., Turkov, D., Schumacher, C., Jacobo, A., McEnerney, A., Ramsey, A., Xu, G., Park, D., Kalomoiris, S., Yao, W., Jao, L.E., Allende, M.L., Lebrilla, C.B., Fierro, F.A.
- Source
- Full text @ Stem Cell Reports
Silencing FUT8 Affects Migration of MSC in Zebrafish and Mice (A) Representative image of GFP-expressing human MSCs around the head of 1-dpf zebrafish. Insert shows high magnification, where protrusions from an individual cell are visible. (B) MSCs distributed around the trunk region. (C) MSCs distributed in the tail. Distribution of transplanted cells is highlighted by arrows. (D) Quantification of cell distribution, where ∗p < 0.05 as assessed using a paired Student's t test (N = 5 independent experiments performed with biological replicates). (E) Seven days after bone fracture and intramuscular injection of cells, mice were analyzed for presence of tdTomato-expressing mouse MSCs. Control cells distribute abundantly in callus and bone marrow of the fractured femur. (F) MSCs with shFUT8 only minimally reach the new callus and bone marrow on fracture site. Error bars indicate standard error of the mean (SEM). Scale bars, 100 μm. |