Feeding amount alters melanoma tumor onset in zebrafish p53/BRAF model. (A) Feeding paradigm used to rapidly rear p53/BRAF melanoma-prone zebrafish. (B) Gross tumor onset reported as Kaplan–Meier curves for percent (%) tumor-free survival at the indicated age (dpf). Number of zebrafish at risk are reported at 50 day intervals based on the most proximal prior observation. Reported P values are based on the Log-rank (Mantel-Cox) Test as determined by Prism software where curves were generated. NR, not reached. (C) Zebrafish length at the indicated times for various adult feeding regimens. SL, snout to caudal peduncle length, is reported in centimeters with mean±standard deviation. (D) Representative images of p53/BRAF zebrafish at 155 dpf in each feeding group. Scale bar: 1 cm.