- Title
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Neural stem cells induce the formation of their physical niche during organogenesis
- Authors
- Seleit, A., Krämer, I., Riebesehl, B.F., Ambrosio, E.M., Stolper, J.S., Lischik, C.Q., Dross, N., Centanin, L.
- Source
- Full text @ Elife
Conservation of nBCs in neuromasts of other teleost fish.
(A) DAPI-stained neuromast Peocilia formosa, a close relative of medaka, reveals the presence of nBCs as assessed by their distinct morphology and relative location (N = 10 neuromasts in a single 1 month old fish). (B) Close-up on a Peocilia formosa neuromast reveals that nBCs are immediately adjacent to mantle cells (N = 10 border cells in four neuromasts). (C) DAPI-stained neuromast of Danio rerio, a distant relative of medaka, reveals the presence of nBCs as assessed by their distinct morphology and location (N=>5 neuromasts in three larvae). (D) Close-up of a zebrafish neuromast reveals that nBCs intimately associate with mantle cells (N=>5 neuromasts in three larvae). (E) Tg(ol.K15::H2B-eGFP) shows labelling of the skin epithelium and mantle cells in a 5 dph zebrafish larvae. (F) Close-up of a Tg(ol.K15::H2B-eGFP) zebrafish neuromast shows labelling of cells within the neural lineage and nBCs (N= 5 neuromasts in three larvae). Scalebars = 10 µm, except in panel E, Scalebar = 100 µm. |