- Title
-
Generating libraries of iTol2-end insertions at BAC ends using loxP and lox511 Tn10 transposons
- Authors
- Shakes, L.A., Abe, G., Eltayeb, M.A., Wolf, H.M., Kawakami, K., and Chatterjee, P.K.
- Source
- Full text @ BMC Genomics
"Excision assay" performed with genomic DNA isolated from 2-day old zebrafish embryos: GFP expression in the embryos injected with iTol2-BACs were observed between 28 and 48 hours post fertilization (hpf) (upper panels). APPb:EGFP BAC 13 (panel 5A), APPb:EGFP BAC 2 (panel 5B), fgf24:EGFP BAC 37 (panel 5C) and fgf24:EGFP BAC 35 (panel 5D) display green fluorescence. The white arrowheads indicate the expected tissue-specific expressions of GFP in these embryos. The iTol2-BAC injected embryos were analyzed using the "excision assay" (lower panels) [16,4]. |
EGFP fluorescence in transgenic F1 zebrafish: Panel A: Germline expression of EGFP fluorescence in zebrafish embryos with DNA from APPb:EGFP BAC clone 15. Panel B shows the germline expression of EGFP fluorescence with DNA from APPb:EGFP BAC 27, and Panel C shows germline expression from fgf24:EGFP BAC clone 37. |