mRNA expression of the pumilio-2 in adult zebrafish tissues. A Analysis of mRNA expression of the pumilio-2 in several zebrafish tissues using RT-PCR. RNA template and β-actin were used as negative and positive controls, respectively. The tissues shown include the ovary, testis, liver, kidney, heart, muscle and brain. B Expression of the zebrafish pumilio-2 in ovary and testis (In situ hybridization). a: Whole in situ hybridization of ovary; b: Ovary slide following in situ hybridization. The nucleus is located in the center of the oocyte; I–IV: Oocyte during phases I–IV; c: Whole in situ hybridization of testis; d: Testis slide after in situ hybridization; e: Enlarged the black rectangle of d. Scale bars: 0.3 mm in all panels

mRNA expression of zebrafish pumilio-2 in the early phases of embryonic development. A RT-PCR: 1-cell to 5-day-old fish. RNA template was used as negative control; β-actin was used as positive control. B Whole-mount in situ hybridization. a: 1-cell stage; b: 2-cell stage; c: 4-cell stage; d: Blastula stage; e: 60%-epiboly; f: 3-somite stage; g: 24 hpf. The arrows point to the distinct dots in the cleavage furrows. Scale bars: 0.2 mm in all panels

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Protein localization and distribution of the zebrafish Pumilio-2 in the ovary as detected by immunohistochemistry. a H&E staining, b DAB immunostained with the Pumillo-2 polyclonal antibody. c DAB immunostained with pre-immunization serum. I: stage I oocyte; II: stage II oocyte; III: stage III oocyte; IV: stage IV oocyte. N: nucleus. Scale bars: 0.3 mm in all panels