- Title
-
Transcriptome analysis of the zebrafish pineal gland
- Authors
- Toyama, R., Chen, X., Jhawar, N., Aamar, E., Epstein, J., Reany, N., Alon, S., Gothilf, Y., Klein, D.C., and Dawid, I.B.
- Source
- Full text @ Dev. Dyn.
unc119c and arl3l2 are co-expressed in the pineal gland and interact with each other. A,B: Spatial expression of zebrafish unc119c (A) and arl3l2 (B). Both panels are shown as dorsal views of 3 dpf larvae. In situ hybridization was carried out as described (Toyama and Dawid,[1997]). C: Co-immunoprecipitation of zebrafish Unc119c and Arl3l2 transfected into HEK293 cells. The antibody used for precipitation is indicated by IP, and the antibodies used for blotting are shown on the right side of the panels. Myc-tagged Arl3l2 co-precipitated with Flag-Unc119c (white arrowhead in the top panel, shown above immunoglobulin light chain.), but Myc-tagged nucleolin (Myc-Nucleo) did not. Black arrowheads indicate Myc-tagged Arl3l2 and Nucleolin (used as negative control) in the middle panel. EXPRESSION / LABELING:
|
Spatial expression pattern of selected pineal enriched genes in day 3 zebrafish larvae. In situ hybridization was carried out as described (Toyama and Dawid, 1997). The Affymetrix probe set ID numbers corresponding to the different clones are indicated. A, Dr.9901.1.S1; B, Dr.6401.1.S1; C, Dr.11336.1.A1; D, Dr.9852.1.A1; E and F, Dr.12466.1.A1. F, eyes are removed. All panels show lateral views. |
Unillustrated author statements EXPRESSION / LABELING:
|