Lab
Pantazis Lab
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Statement of Research Interest
The Laboratory of NanoBioImaging combines cutting-edge imaging technologies with sophisticated quantitative imaging analyses to investigate early development and tissue regeneration in vivo. For this purpose, we apply confocal and non-linear imaging modalities, including two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) imaging.
We invented SHG nanoprobes as superior imaging probes, enabling high contrast time-lapse imaging deep within tissues without any signal decay over time. Since their optical signal is based on scattering, rather than absorption, they neither bleach nor blink, and their signal does not saturate. They can be detected with little background signal and are physiologically inert. We are currently pioneering uses of these nanolabels for clinically relevant applications.
In another line of research, we developed the so-called PhOTO zebrafish. PhOTO zebrafish combine the benefits of global and sparse embryo labeling for cell tracking in the early embryo and extends this ability to monitor the contribution of labeled cells during adult stage epimorphic regeneration.
Finally, we established a quantitative imaging assay, the Fluoresence Decay After Photoactivation (FDAP) assay, as an optimal tool for dissecting early pluripotency decisions in developing mammalian embryos.
We invented SHG nanoprobes as superior imaging probes, enabling high contrast time-lapse imaging deep within tissues without any signal decay over time. Since their optical signal is based on scattering, rather than absorption, they neither bleach nor blink, and their signal does not saturate. They can be detected with little background signal and are physiologically inert. We are currently pioneering uses of these nanolabels for clinically relevant applications.
In another line of research, we developed the so-called PhOTO zebrafish. PhOTO zebrafish combine the benefits of global and sparse embryo labeling for cell tracking in the early embryo and extends this ability to monitor the contribution of labeled cells during adult stage epimorphic regeneration.
Finally, we established a quantitative imaging assay, the Fluoresence Decay After Photoactivation (FDAP) assay, as an optimal tool for dissecting early pluripotency decisions in developing mammalian embryos.
Lab Members