Mapping Details

GENETIC MAPPING PANELS

Chr	Location	Mapped As	Panel	Mapped By	Scoring
2	46.6 cM	z22527	Boston MGH Cross (MGH)	Fishman, Mark C.	Data
2	412.29 cR	Z22527	Loeb/NIH/5000/4000 (LN54)	Dawid, Igor B.	Data
2	2766.0 cR	z22527	Goodfellow T51 (T51)	Geisler, Robert	Data
2	89.7 cM	z22527	Heat Shock (HS)	Woods, Ian G.	Data
Note: Physical map location as displayed in the genome browsers is more precise than linkage map location. Note: Physical map location should be used whenever possible.

MAPPING FROM PUBLICATIONS

Marker	Type	Chr	Distance	Publication / Person	Comments
z7632	SSLP	2		Cui et al., 2005	Cui et al (J. Neurosci, 25:6610-6620) used bulk segregant analysis to map the  ... Cui et al (J. Neurosci, 25:6610-6620) used bulk segregant analysis to map the sho locus to LG 2. The closest flanking markers were z22527 and z7632. Subsequent analyses using data from the zebrafish fingerprinting project and genome sequence identified a missense mutation in the slc6a9 gene to be the cause of the sho mutation.
ta229g	Feature	2	0.97 cM	Cui et al., 2005	Cui et al (J. Neurosci, 25:6610-6620) used bulk segregant analysis to map the  ... Cui et al (J. Neurosci, 25:6610-6620) used bulk segregant analysis to map the sho locus to LG 2. The closest flanking markers were z22527 and z7632. Subsequent analyses using data from the zebrafish fingerprinting project and genome sequence identified a missense mutation in the slc6a9 gene to be the cause of the sho mutation.
slc6a9	GENE	2		Cui et al., 2005	Cui et al (J. Neurosci, 25:6610-6620) used bulk segregant analysis to map the  ... Cui et al (J. Neurosci, 25:6610-6620) used bulk segregant analysis to map the sho locus to LG 2. The closest flanking markers were z22527 and z7632. Subsequent analyses using data from the zebrafish fingerprinting project and genome sequence identified a missense mutation in the slc6a9 gene to be the cause of the sho mutation.

OTHER MAPPING INFORMATION

Primer Sets:

Strain	Bandsize	Restriction Enzyme	Annealing Temperature [C]
AB	111,0,113		60.0
Forward Primer	GGTGTAGTTTGTGCCAGCCT
Reverse Primer	TGCTAAAGCTGTCTACGCGA
IND	0,119		60.0
Forward Primer	GGTGTAGTTTGTGCCAGCCT
Reverse Primer	TGCTAAAGCTGTCTACGCGA
EKW	0,111		60.0
Forward Primer	GGTGTAGTTTGTGCCAGCCT
Reverse Primer	TGCTAAAGCTGTCTACGCGA
TU	0,111		60.0
Forward Primer	GGTGTAGTTTGTGCCAGCCT
Reverse Primer	TGCTAAAGCTGTCTACGCGA