Transgenic Construct

Tg2(Xla.Rho:crb2a-HA)

ID

ZDB-TGCONSTRCT-110131-35

Previous Names

Extra_Secr (1)
Tg(Xla.Rho:crb2.2-HA) (1)
Tg(Xla.Rho:crb2a.2-HA)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

crb2a (1)
HA (1)

Contains Sequences

HA (1)

Note

This construct utilizes 0.8 kB of the Xenopus rod opsin (Rho) promoter with an N terminal signal peptide taken from zebrafish Crb2b and an influenza hamagglutinin (HA) tag (YPYDVPDYA). To make the Extra_Secr construct, site directed mutagenesis was used to place a Stop codon just before the transmembrane domain.

Plasmid Map

None

Genomic Features

That Utilize Tg2(Xla.Rho:crb2a-HA) Construct

Genomic Feature	Affected Genomic Regions
umz7Tg
umz8Tg
umz9Tg

Transgenics

That Utilize Tg2(Xla.Rho:crb2a-HA) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
slc45a2^b4/+; fl1Tg/+; umz7Tg/+	slc45a2
slc45a2^b4/+; fl1Tg/+; umz8Tg/+	slc45a2
slc45a2^b4/b4; fl1Tg; umz7Tg	slc45a2	Fig. 4 from Hsu et al., 2010	7 figures from Hsu et al., 2010
slc45a2^b4/b4; fl1Tg; umz8Tg	slc45a2
slc45a2^b4/b4; fl1Tg; umz9Tg	slc45a2
umz9Tg

Sequence Information

Other Construct Pages

None

Citations