PUBLICATION

Transient establishment of anteroposterior polarity in the zebrafish pectoral fin bud in the absence of sonic hedgehog activity

Authors

Neumann, C.J., Grandel, H., Gaffield, W., Schulte-Merker, S., Nüsslein-Volhard, C.

ID

ZDB-PUB-991103-2

Date

1999

Source

Development (Cambridge, England) 126(21): 4817-4826 (Journal)

Registered Authors

Grandel, Heiner, Neumann, Carl J., Nüsslein-Volhard, Christiane, Schulte-Merker, Stefan

Keywords

sonic hedgehog; limb; fin; ZPA; hox; colinearity; cyclopamine; retinoic acid; zebrafish; Danio rerio

MeSH Terms

Animals
Body Patterning/physiology*
Bone Morphogenetic Protein 2
Bone Morphogenetic Proteins/genetics
Cell Division/genetics
DNA-Binding Proteins/genetics
Ectoderm
Embryo, Nonmammalian/cytology
Embryo, Nonmammalian/drug effects
Gene Expression Regulation, Developmental
Hedgehog Proteins
Homeodomain Proteins/genetics
Larva
Limb Buds/cytology
Limb Buds/growth & development*
Membrane Proteins/genetics
Mutation
Proteins/genetics*
Proteins/metabolism
Receptors, Cell Surface
Trans-Activators*
Transcription Factors/genetics
Transforming Growth Factor beta*
Tretinoin/pharmacology
Veratrum Alkaloids/pharmacology
Zebrafish/embryology*
Zebrafish Proteins*

(all 27)

PubMed

10518498 Full text @ Development

Abstract

Sonic hedgehog (Shh) is expressed in the posterior vertebrate limb bud mesenchyme and directs anteroposterior patterning and growth during limb development. Here we report an analysis of the pectoral fin phenotype of zebrafish sonic you mutants, which disrupt the shh gene. We show that Shh is required for the establishment of some aspects of anteroposterior polarity, while other aspects of anteroposterior polarity are established independently of Shh, and only later come to depend on Shh for their maintenance. We also demonstrate that Shh is required for the activation of posterior HoxD genes by retinoic acid. Finally, we show that Shh is required for normal development of the apical ectodermal fold, for growth of the fin bud, and for formation of the fin endoskeleton.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
Df(Chr07)t4	standard conditions	Prim-15	apical ectodermal ridge pectoral fin bud absent, abnormal	Fig. 7
Df(Chr07)t4	standard conditions	Prim-15	pectoral fin bud decreased size, abnormal	Fig. 7
Df(Chr07)t4	standard conditions	Prim-15	pectoral fin bud portion of tissue poorly differentiated, abnormal	Fig. 7
Df(Chr07)t4	standard conditions	Prim-25	apical ectodermal ridge pectoral fin bud absent, abnormal	Fig. 7
Df(Chr07)t4	standard conditions	Prim-25	pectoral fin bud cell population proliferation decreased frequency, abnormal	Fig. 8
Df(Chr07)t4	standard conditions	Prim-25	pectoral fin bud decreased size, abnormal	Fig. 7
Df(Chr07)t4	standard conditions	Prim-25	pectoral fin bud portion of tissue poorly differentiated, abnormal	Fig. 7
Df(Chr07)t4	standard conditions	Prim-25	pectoral fin fold absent, abnormal	Fig. 7
Df(Chr07)t4	standard conditions	Protruding-mouth	pectoral fin bud decreased size, abnormal	Fig. 7
Df(Chr07)t4	standard conditions	Protruding-mouth	pectoral fin bud portion of tissue poorly differentiated, abnormal	Fig. 7

1 - 10 of 21

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
t4		Deficiency	shha
tq252		Point Mutation	shha

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Neumann et al., 1999 ZDB-PUB-991103-2 PMID:10518498

Transient establishment of anteroposterior polarity in the zebrafish pectoral fin bud in the absence of sonic hedgehog activity

Neumann et al., 1999

ZDB-PUB-991103-2
PMID:10518498