PUBLICATION
ß-lactamase as a marker for gene expression in live zebrafish embryos
- Authors
- Raz, E., Zlokarnik, G., Tsien, R.Y., and Driever, W.
- ID
- ZDB-PUB-981208-28
- Date
- 1998
- Source
- Developmental Biology 203: 290-294 (Journal)
- Registered Authors
- Driever, Wolfgang, Raz, Erez
- Keywords
- zebrafish; ß-lactamase; dominant marker
- MeSH Terms
-
- Animals
- Biomarkers*
- Fluoresceins/metabolism
- Fluorescence
- Fluorescent Dyes/metabolism
- Gene Expression Regulation, Developmental/genetics*
- Gene Expression Regulation, Enzymologic/genetics
- Genes, Reporter/genetics
- Green Fluorescent Proteins
- Lactams*
- Luminescent Proteins/genetics
- Zebrafish/embryology*
- beta-Lactamases/genetics*
- beta-Lactams/metabolism
- PubMed
- 9808780 Full text @ Dev. Biol.
Citation
Raz, E., Zlokarnik, G., Tsien, R.Y., and Driever, W. (1998) ß-lactamase as a marker for gene expression in live zebrafish embryos. Developmental Biology. 203:290-294.
Abstract
In this report we describe the development of a sensitive assay for gene expression in zebrafish embryos using beta-lactamase as a reporter gene. We show that injection of a green fluorescent substrate for beta-lactamase allows the detection of reporter gene expression in live embryos. The beta-lactamase enzyme catalyzes the hydrolysis of the substrate, thereby disrupting fluorescence resonance energy transfer from the donor to the acceptor dye in the molecule. As a result, a blue fluorescent product is produced and retained specifically in cells within which the enzyme is expressed. beta-Lactamase is therefore suitable for monitoring spatially restricted patterns of gene expression in the early embryo. We suggest that this new reporter system provides a major advancement in sensitivity over the existing methods for monitoring gene expression in vivo during early embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping