PUBLICATION
Molecular identification of spadetail: regulation of zebrafish trunk and tail mesoderm formation by T-box genes
- Authors
- Griffin, K.J.P., Amacher, S.L., Kimmel, C.B., and Kimelman, D.
- ID
- ZDB-PUB-980820-3
- Date
- 1998
- Source
- Development (Cambridge, England) 125: 3379-3388 (Journal)
- Registered Authors
- Amacher, Sharon, Griffin, Kevin, Kimelman, David, Kimmel, Charles B.
- Keywords
- spadetail; brachyury; no tail; tbx6; posterior development; FGF; zebrafish; T-box
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA Primers/genetics
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/physiology
- Fetal Proteins*
- Fibroblast Growth Factors/physiology
- Gene Expression Regulation, Developmental
- In Situ Hybridization
- Mesoderm/cytology
- Mesoderm/physiology
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Phenotype
- Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Signal Transduction
- T-Box Domain Proteins
- Tail/embryology
- Transcription Factors/genetics*
- Transcription Factors/physiology
- Vertebrates/embryology
- Vertebrates/genetics
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/physiology
- Zebrafish Proteins*
- PubMed
- 9693141 Full text @ Development
Citation
Griffin, K.J.P., Amacher, S.L., Kimmel, C.B., and Kimelman, D. (1998) Molecular identification of spadetail: regulation of zebrafish trunk and tail mesoderm formation by T-box genes. Development (Cambridge, England). 125:3379-3388.
Abstract
Inhibition of fibroblast growth factor (FGF) signaling prevents trunk and tail formation in Xenopus and zebrafish embryos. While the T-box transcription factor Brachyury (called no tail in zebrafish) is a key mediator of FGF signaling in the notochord and tail, the pathways activated by FGF in non-notochordal trunk mesoderm have been uncertain. Previous studies have shown that the spadetail gene is required for non-notochordal trunk mesoderm formation; spadetail mutant embryos have major trunk mesoderm deficiencies, but relatively normal tail and notochord development. We demonstrate here that spadetail encodes a T-box transcription factor with homologues in Xenopus and chick. Spadetail is likely to be a key mediator of FGF signaling in trunk non-notochordal mesoderm, since spadetail expression is regulated by FGF signaling. Trunk and tail development are therefore dependent upon the complementary actions of two T-box genes, spadetail and no tail. We show that the regulatory hierarchy among spadetail, no tail and a third T-box gene, tbx6, are substantially different during trunk and tail mesoderm formation, and propose a genetic model that accounts for the regional phenotypes of spadetail and no tail mutants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping