PUBLICATION

Sonic hedgehog is not required for the induction of medial floor plate cells in the zebrafish

Authors
Schauerte, H.E., van Eeden, F.J.M., Fricke, C., Odenthal, J., Strähle, U., Haffter, P.
ID
ZDB-PUB-980729-15
Date
1998
Source
Development (Cambridge, England)   125: 2983-2993 (Journal)
Registered Authors
Fricke, Cornelia, Haffter, Pascal, Odenthal, Joerg, Schauerte, Heike, Strähle, Uwe, van Eeden, Freek
Keywords
sonic hedgehog; sonic-you; zebrafish; floor plate; somitogenesis
MeSH Terms
  • Animals
  • Axons
  • Body Patterning
  • Cyclic AMP-Dependent Protein Kinases/metabolism
  • DNA-Binding Proteins/biosynthesis
  • Embryonic Induction*
  • Eye/embryology
  • Eye Proteins
  • Genetic Complementation Test
  • Hedgehog Proteins
  • Homeodomain Proteins/biosynthesis
  • Muscles/cytology
  • Mutation
  • Nervous System/embryology*
  • Nervous System Malformations
  • PAX2 Transcription Factor
  • Paired Box Transcription Factors
  • Protein Sorting Signals/genetics
  • Proteins/metabolism*
  • RNA Splicing
  • Repressor Proteins
  • Retinal Ganglion Cells
  • Sequence Analysis, DNA
  • Signal Transduction
  • Somites*
  • Stem Cells
  • Trans-Activators*
  • Transcription Factors/biosynthesis
  • Zebrafish/embryology*
  • Zebrafish Proteins
PubMed
9655820 Full text @ Development
Abstract
Sonic hedgehog (Shh) is a secreted protein that is involved in the organization and patterning of several tissues in vertebrates. We show that the zebrafish sonic-you (syu) gene, a member of a group of five genes required for somite patterning, is encoding Shh. Embryos mutant for a deletion of syu display defects in patterning of the somites, the lateral floor plate cells, the pectoral fins, the axons of motorneurons and the retinal ganglion cells. In contrast to mouse embryos lacking Shh activity, syu mutant embryos do form medial floor plate cells and motorneurons. Since ectopic overexpression of shh in zebrafish embryos does not induce ectopic medial floor plate cells, we conclude that shh is neither required nor sufficient to induce this cell type in the zebrafish.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping