PUBLICATION

Cloning, molecular characterization, and distribution of a gene homologous to delta opioid receptor from zebrafish (Danio rerio)

Authors
Barrallo, A., González Sarmiento, R., Porteros, A., García Isidoro, M., and Rodriguez, R.E.
ID
ZDB-PUB-980514-3
Date
1998
Source
Biochemical and Biophysical Research Communications   245: 544-548 (Journal)
Registered Authors
Barrallo Gimeno, Alejandro, García Isidoro, María, González Sarmiento, Rogelio, Rodriguez, Raquel E.
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Brain/metabolism
  • Cloning, Molecular
  • Conserved Sequence/genetics
  • Evolution, Molecular
  • Exons/genetics
  • In Situ Hybridization
  • Molecular Sequence Data
  • RNA, Messenger/analysis
  • Receptors, Opioid, delta/chemistry*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Zebrafish
PubMed
9571192 Full text @ Biochem. Biophys. Res. Commun.
Abstract
A full-length cDNA, ZFOR1, has been isolated from the teleost zebrafish (Danio rerio) using a probe from rat mu opioid receptor. ZFOR1 encodes a 373 amino acid protein with seven potential transmembrane domains that shows a high degree of homology to mammalian delta opioid receptor. We have also isolated a genomic clone which contains two exons of ZFOR1, homologous to exons 2 and 3 in mouse and human delta opioid receptor. Expression of ZFOR1 appears to be restricted to nervous tissue as assessed by Northern blot. In situ hybridization in zebrafish brain with specific probes revealed several discrete areas of ZFOR1 expression; higher levels are detected in dorsal telencephalic areas, the periventricular layer of the optic tectum, and the granular layer of the cerebellum. This is the first molecular evidence of the presence of the delta opioid receptor in a non-mammalian species and suggests that it has been highly conserved throughout vertebrate evolution.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping