PUBLICATION

Neuronal and neuroendocrine expression of lim3, a LIM class homeobox gene, is altered in mutant zebrafish with axial signaling defects

Authors
Glasgow, E., Karavanov, A.A., and Dawid, I.B.
ID
ZDB-PUB-980205-18
Date
1997
Source
Developmental Biology   192: 405-419 (Journal)
Registered Authors
Dawid, Igor B., Glasgow, Eric
Keywords
pituitary, pineal, spinal cord, development, transcription factors
MeSH Terms
  • Abnormalities, Multiple/genetics
  • Amino Acid Sequence
  • Animals
  • Cell Lineage
  • Central Nervous System/abnormalities
  • Central Nervous System/embryology
  • Central Nervous System/metabolism
  • Gene Expression Regulation, Developmental*
  • Genes, Homeobox*
  • Head/embryology
  • Homeodomain Proteins/biosynthesis*
  • Homeodomain Proteins/genetics
  • LIM-Homeodomain Proteins
  • Molecular Sequence Data
  • Nerve Tissue Proteins/biosynthesis*
  • Nerve Tissue Proteins/genetics
  • Neurons/metabolism*
  • Neurosecretory Systems/metabolism*
  • Pituitary Gland/embryology
  • Pituitary Gland/metabolism
  • RNA, Messenger/biosynthesis
  • Rhombencephalon/embryology
  • Rhombencephalon/metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Spinal Cord/embryology
  • Spinal Cord/metabolism
  • Tail/embryology
  • Transcription Factors
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins*
(all 32)
PubMed
9441677 Full text @ Dev. Biol.
Abstract
LIM class homeobox genes code for a family of transcriptional regulators that encode important determinants of cell lineage and cell type specificity. The lim3 gene from the zebrafish, Danio rerio, is highly conserved in sequence and expression pattern compared to its homologs in other vertebrates. In this paper we report immunocytochemical analysis of Lim3 protein expression in the pituitary, pineal, hindbrain, and spinal cord of the embryo, revealing an asymmetrical, lateral and late program of pituitary development in zebrafish, distinct from the pattern in other vertebrates. We studied Lim3 expression in no tail, floating head, and cyclops mutant embryos, all of which have midline defects, with special reference to spinal cord differentiation where Lim3 marks mostly motoneurons. cyclops embryos showed essentially normal Lim3 expression in the hindbrain and spinal cord despite the absence of the floor plate, while no tail mutant embryos, which lack a differentiated notochord, displayed an excess of Lim3-expressing cells in a generally normal pattern. In contrast, Lim3-positive cells largely disappeared from the posterior spinal cord in floating head mutants, except in patches that correlated with remnants of apparent floor plate cells. These results support the view that either notochord or floor plate signaling can specify Lim3-positive motoneurons in the spinal cord.
Genes / Markers
Marker Marker Type Name
lhx3GENELIM homeobox 3
ndr2GENEnodal-related 2
notoGENEnotochord homeobox
tbxtaGENET-box transcription factor Ta
1 - 4 of 4
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Figures
Figure Gallery (6 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
noton1/n1standard conditionsPrim-5
noton1/n1standard conditionsPrim-5
1 - 2 of 2
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b16
    		Deficiency
    b160
      		Indel
      n1
        		Small Deletion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        Df(Chr12:hhex,ndr2)b16/b16
        noton1/n1
        tbxtab160/b160
        WT
        1 - 4 of 4
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        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available
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        Citation
        Glasgow, E., Karavanov, A.A., and Dawid, I.B. (1997) Neuronal and neuroendocrine expression of lim3, a LIM class homeobox gene, is altered in mutant zebrafish with axial signaling defects. Developmental Biology. 192:405-419.