PUBLICATION
Cloning, sequencing, and embryonic expression of an N-ras proto-oncogene isolated from an enriched zebrafish (Danio rerio) cDNA library
- Authors
- Cheng, R., Bradford, S., Barnes, D., Williams, D., Hendricks, J., and Bailey, G.
- ID
- ZDB-PUB-970918-22
- Date
- 1997
- Source
- Molecular marine biology and biotechnology 6(1): 40-47 (Journal)
- Registered Authors
- Bailey, George, Barnes, David W., Bradford, Sam, Williams, Darren W.
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA Primers
- DNA, Complementary
- Embryo, Nonmammalian
- Exons
- Gene Expression Regulation, Developmental
- Gene Library
- Genes, ras*
- Humans
- Mice
- Molecular Sequence Data
- Polymerase Chain Reaction
- Proto-Oncogene Proteins p21(ras)/biosynthesis
- Proto-Oncogene Proteins p21(ras)/genetics*
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- Transcription, Genetic
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 9116869
Citation
Cheng, R., Bradford, S., Barnes, D., Williams, D., Hendricks, J., and Bailey, G. (1997) Cloning, sequencing, and embryonic expression of an N-ras proto-oncogene isolated from an enriched zebrafish (Danio rerio) cDNA library. Molecular marine biology and biotechnology. 6(1):40-47.
Abstract
An enriched zebrafish (Danio rerio) complementary DNA library was constructed for screening of ras-related genes, and a positive clone was isolated from one plate of 3 x 10(4) plaques. This clone, Zras-B1, carried an insert of 2592 base pairs (bp) with an open reading frame encoding a ras p21 protein of 188 amino acids. The deduced amino-terminal 86 amino acid residues and the carboxy-terminal CAAX binding motif are identical to mammalian ras. The full-length Zras-B1-encoded protein is most closely related to human N-ras (91% identity), with lesser homology to Ha-ras (84%) and Ki-ras (85%). Preliminary screening data also indicate other ras genes in zebrafish, at least one of which is also transcribed in adults. A Zras-B1-related 3.1-kb transcript was found to be abundant in embryos from zygote through gastrulation, and may be maternally derived.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping