PUBLICATION

dino and mercedes, two genes regulating dorsal development in the zebrafish embryo

Authors

Hammerschmidt, M., Pelegri, F., Mullins, M.C., Kane, D.A., van Eeden, F.J., Granato, M., Brand, M., Furutani-Seiki, M., Haffter, P., Heisenberg, C.P., Jiang, Y.J., Kelsh, R.N., Odenthal, J., Warga, R.M., and Nüsslein-Volhard, C.

ID

ZDB-PUB-970210-8

Date

1996

Source

Development (Cambridge, England) 123: 95-102 (Journal)

Registered Authors

Brand, Michael, Furutani-Seiki, Makoto, Granato, Michael, Haffter, Pascal, Hammerschmidt, Matthias, Heisenberg, Carl-Philipp, Jiang, Yun-Jin, Kane, Donald A., Kelsh, Robert, Mullins, Mary C., Nüsslein-Volhard, Christiane, Odenthal, Joerg, Pelegri, Francisco, van Eeden, Freek, Warga, Rachel M.

Keywords

zebrafish; dorsalization; mesoderm patterning; neural induction; dino; mercedes

MeSH Terms

Animals
Embryonic Development
Gastrula/physiology
Gene Expression Regulation, Developmental*
Genes*
Mesoderm/physiology
Mutagenesis
Zebrafish/embryology
Zebrafish/genetics*
Zebrafish/growth & development*

(all 10)

PubMed

9007232 Full text @ Development

Abstract

We describe two genes, dino and mercedes, which are required for the organization of the zebrafish body plan. In dino mutant embryos, the tail is enlarged at the expense of the head and the anterior region of the trunk. The altered expression patterns of various marker genes reveal that, with the exception of the dorsal most marginal zone, all regions of the early dino mutant embryo acquire more ventral fates. These alterations are already apparent before the onset of gastrulation. mercedes mutant embryos show a similar but weaker phenotype, suggesting a role in the same patterning processes. The phenotypes suggests that dino and mercedes are required for the establishment of dorsal fates in both the marginal and the animal zone of the early gastrula embryo. Their function in the patterning of the ventrolateral mesoderm and the induction of the neuroectoderm is similar to the function of the Spemann organizer in the amphibian embryo.

Genes / Markers

Marker	Marker Type	Name
chrd	GENE	chordin
eve1	GENE	even-skipped-like1
foxa3	GENE	forkhead box A3
foxb1a	GENE	forkhead box B1a
gata1a	GENE	GATA binding protein 1a
gata2a	GENE	GATA binding protein 2a
gsc	GENE	goosecoid
myod1	GENE	myogenic differentiation 1
pax2a	GENE	paired box 2a
shha	GENE	sonic hedgehog signaling molecule a

1 - 10 of 13

Show

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
chrd^tm84/tm84	standard conditions	30%-epiboly to 75%-epiboly	dorsal/ventral pattern formation disrupted, abnormal	Fig. 2
chrd^tm84/tm84	standard conditions	30%-epiboly to 75%-epiboly	whole organism wholly ventralized, abnormal	Fig. 2
chrd^tm84/tm84	standard conditions	Bud	dorsal/ventral pattern formation disrupted, abnormal	Fig. 1
chrd^tm84/tm84	standard conditions	Bud	hatching gland size, normal	Fig. 1
chrd^tm84/tm84	standard conditions	Bud	head decreased thickness, abnormal	Fig. 1
chrd^tm84/tm84	standard conditions	Bud	prechordal plate size, normal	Fig. 1
chrd^tm84/tm84	standard conditions	1-4 somites	somite 1 decreased size, abnormal	Fig. 1
chrd^tm84/tm84	standard conditions	1-4 somites	somite 2 decreased size, abnormal	Fig. 1
chrd^tm84/tm84	standard conditions	1-4 somites	somite 3 decreased size, abnormal	Fig. 1
chrd^tm84/tm84	standard conditions	Bud	tail bud increased thickness, abnormal	Fig. 1

1 - 10 of 131

Show

Mutations / Transgenics

Allele	Type	Affected Genomic Region
tm84	Unknown	chrd
tm305	Point Mutation	szl
tt250	Point Mutation	chrd
tz209	Point Mutation	szl

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
chrd^tm84/tm84
chrd^tt250/tt250
szl^tm305/+; chrd^tm84/+
szl^tm305/tm305
szl^tm305/tm305; chrd^tm84/tm84
szl^tz209/tz209
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Hammerschmidt et al., 1996 ZDB-PUB-970210-8 PMID:9007232

dino and mercedes, two genes regulating dorsal development in the zebrafish embryo

Hammerschmidt et al., 1996

ZDB-PUB-970210-8
PMID:9007232