PUBLICATION

valentino, a zebrafish gene required for normal hindbrain segmentation

Authors
Moens, C.B., Yan, Y.L., Appel, B., Force, A.G., and Kimmel, C.B.
ID
ZDB-PUB-970127-5
Date
1996
Source
Development (Cambridge, England)   122: 3981-3990 (Journal)
Registered Authors
Appel, Bruce, Force, Allan G., Kimmel, Charles B., Moens, Cecilia, Yan, Yi-Lin
Keywords
zebrafish; hindbrain; segmentation; valentino; rhombomere
MeSH Terms
  • Animals
  • Avian Proteins*
  • Body Patterning/genetics*
  • Cell Transplantation
  • DNA-Binding Proteins/genetics
  • Genetic Markers
  • In Situ Hybridization
  • Maf Transcription Factors
  • Models, Biological
  • Mosaicism
  • Oncogene Proteins*
  • Polymerase Chain Reaction
  • RNA, Messenger/isolation & purification
  • Rhombencephalon/anatomy & histology
  • Rhombencephalon/embryology*
  • Selection, Genetic
  • Time Factors
  • Transcription Factors/genetics
  • Zebrafish/embryology
  • Zebrafish Proteins
(all 20)
PubMed
9012518 Full text @ Development
Abstract
Mutational analysis can serve both to identify new genes essential for patterning embryonic development and to determine their functions. Here we describe the identification and phenotypic characterization of alleles of valentino, which we recovered in a genetic screen that sought to identify mutations in the zebrafish that disrupt region-specific gene expression patterns in the embryonic brain. valentino is required for normal hindbrain segmentation and the hindbrain of valentino mutant embryos is shortened by the length of one rhombomere. We demonstrate that valentino is required cell-autonomously in the development of rhombomeres 5 and 6, and propose that valentino functions in the subdivision and expansion of a common precursor region in the presumptive hindbrain into the definitive rhombomeres 5 and 6. These results provide genetic evidence for a two-segment periodicity in the hindbrain and suggest that this periodicity arises sequentially, through the specification and later subdivision of a two-rhombomere unit, or 'protosegment'.
Genes / Markers
Marker Marker Type Name
dlx2aGENEdistal-less homeobox 2a
egr2bGENEearly growth response 2b
en2bGENEengrailed homeobox 2b
foxb1aGENEforkhead box B1a
gap43GENEgrowth associated protein 43
lhx5GENELIM homeobox 5
mafbaGENEMAF bZIP transcription factor Ba
myod1GENEmyogenic differentiation 1
shhaGENEsonic hedgehog signaling molecule a
snai1bGENEsnail family zinc finger 1b
1 - 10 of 10
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Figures
Figure Gallery (4 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
mafbab337/b337 (AB)standard conditions14-19 somites
mafbab337/b337 (AB)standard conditions14-19 somites
mafbab337/b337 (AB)standard conditions14-19 somites
mafbab337/b337 (AB)standard conditions26+ somites
mafbab337/b337 (AB)standard conditionsLong-pec
mafbab337/b337 (AB)standard conditionsDay 5
1 - 6 of 6
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b337
    		Point Mutation
    b361
      		Deficiency
      b475
        		Deficiency
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        Df(Chr23:mafba,snai1b)b361/b361 (AB)
        Df(Chr23:mafba,snai1b,tnnt2a)b475/b475 (AB)
        mafbab337/b337 (AB)
        WT
        1 - 4 of 4
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        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available
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        Citation
        Moens, C.B., Yan, Y.L., Appel, B., Force, A.G., and Kimmel, C.B. (1996) valentino, a zebrafish gene required for normal hindbrain segmentation. Development (Cambridge, England). 122:3981-3990.