PUBLICATION

Mutations affecting pigmentation and shape of the adult zebrafish

Authors: Haffter, P., Odenthal, J., Mullins, M.C., Lin, S., Farrell, M.J., Vogelsang, E., Haas, F., Brand, M., van Eeden, F.J.M., Furutani-Seiki, M., Granato, M., Hammerschmidt, M., Heisenberg, C.P., Jiang, Y.J., Kane, D.A., Kelsh, R.N., Hopkins, N., and Nüsslein-Volhard, C.
ID: ZDB-PUB-970108-4
Date: 1996
Source: Development genes and evolution 206(4): 260-276 (Journal)
Registered Authors: Brand, Michael, Farrell, Michael, Furutani-Seiki, Makoto, Granato, Michael, Haffter, Pascal, Hammerschmidt, Matthias, Heisenberg, Carl-Philipp, Hopkins, Nancy, Jiang, Yun-Jin, Kane, Donald A., Kelsh, Robert, Lin, Shuo, Mullins, Mary C., Nüsslein-Volhard, Christiane, Odenthal, Joerg, van Eeden, Freek
Keywords: pigment pattern; fin; fish skeleton; tyrosinase; zebrafish
MeSH Terms: none
PubMed: 24173565 Full text @ Dev. Genes Evol.

Abstract

Mutations causing a visible phenotype in the adult serve as valuable visible genetic markers in multicellular genetic model organisms such as Drosophila melanogaster, Caenorhabditis elegans and Arabidopsis thaliana. In a large scale screen for mutations affecting early development of the zebrafish, we identified a number of mutations that are homozygous viable or semiviable. Here we describe viable mutations which produce visible phenotypes in the adult fish. These predominantly affect the fins and pigmentation, but also the eyes and body length of the adult. A number of dominant mutations caused visible phenotypes in the adult fish. Mutations in three genes, long fin, another long fin and wanda affected fin formation in the adult. Four mutations were found to cause a dominant reduction of the overall body length in the adult. The adult pigment pattern was found to be changed by dominant mutations in wanda, asterix, obelix, leopard, salz and pfeffer. Among the recessive mutations producing visible phenotypes in the homozygous adult, a group of mutations that failed to produce melanin was assayed for tyrosinase activity. Mutations in sandy produced embryos that failed to express tyrosinase activity. These are potentially useful for using tyrosinase as a marker for the generation of transgenic lines of zebrafish.

Genes / Markers

Marker	Marker Type	Name
ase	GENE	asterix
bed	GENE	bedimmed
bmp2b	GENE	bone morphogenetic protein 2b
brs	GENE	brass
bry	GENE	brassy
bum	GENE	bumper
csf1ra	GENE	colony stimulating factor 1 receptor, a
dml	GENE	daeumling
drp	GENE	dropje
edar	GENE	ectodysplasin A receptor

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Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
ase^tt269/+ (AB)	standard conditions	Adult	anal fin has fewer parts of type melanophore stripe, abnormal	Fig. 7
ase^tt269/+ (AB)	standard conditions	Adult	caudal fin has fewer parts of type melanophore stripe, abnormal	Fig. 5
ase^tt269/+ (AB)	standard conditions	Adult	melanophore stripe decreased amount, abnormal	Fig. 5
ase^tt269/+ (AB)	standard conditions	Adult	melanophore stripe decreased amount, abnormal	Fig. 6
ase^tt269/+ (AB)	standard conditions	Adult	melanophore stripe increased width, abnormal	Fig. 5
ase^tt269/+ (AB)	standard conditions	Adult	melanophore stripe increased width, abnormal	Fig. 6
ase^tt269/tt269	standard conditions	Adult	melanophore stripe decreased amount, abnormal	text only
bmp2b^ta72a/+	standard conditions	Adult	whole organism decreased length, abnormal	Table 1
bum^{tm127d/tm127d}	standard conditions	Adult	lens degenerate, abnormal	Fig. 8
csf1ra^tq211/+	standard conditions	Adult	anal fin melanophore stripe patchy, abnormal	Fig. 7

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
b1	Point Mutation	slc24a5
b2	Unknown	brs
b4	Insertion	slc45a2
b5	Small Deletion	kita
b13	Deficiency	slc24a5
b18	Small Deletion	mpv17
b134	Unknown	kita
b140	Unknown	ednrba
dt2	Unknown	lof
dtq270	Point Mutation	gja5b

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
ase^tt269/+ (AB)
ase^tt269/tt269
bed^to240
bmp2b^ta72a/+
brs^b2 (AB)
bry^tm111
bum^tg413
bum^tm127d
bum^{tm127d/tm127d}
bum^to20

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Haffter et al., 1996 ZDB-PUB-970108-4 PMID:24173565

Mutations affecting pigmentation and shape of the adult zebrafish

Haffter et al., 1996

ZDB-PUB-970108-4
PMID:24173565