PUBLICATION

The chinless mutation and neural crest cell interactions in zebrafish jaw development

Authors
Schilling, T.F., Walker, C., and Kimmel, C.B.
ID
ZDB-PUB-961014-987
Date
1996
Source
Development (Cambridge, England)   122: 1417-1426 (Journal)
Registered Authors
Kimmel, Charles B., Schilling, Tom, Walker, Charline
Keywords
none
MeSH Terms
  • Animals
  • Branchial Region/embryology
  • Cartilage/embryology
  • Cell Communication*
  • Cell Lineage
  • Embryonic Induction
  • Jaw/embryology*
  • Mesoderm/physiology
  • Mosaicism
  • Musculoskeletal System/embryology
  • Mutation*
  • Neural Crest/cytology
  • Neural Crest/embryology*
  • Time Factors
  • Tissue Transplantation
  • Zebrafish/embryology*
  • Zebrafish/genetics
(all 17)
PubMed
8625830 Full text @ Development
Abstract
During vertebrate development, neural crest cells are thought to pattern many aspects of head organization, including the segmented skeleton and musculature of the jaw and gills. Here we describe mutations at the gene chinless, chn, that disrupt the skeletal fates of neural crest cells in the head of the zebrafish and their interactions with muscle precursors. chn mutants lack neural-crest-derived cartilage and mesoderm- derived muscles in all seven pharyngeal arches. Fate mapping and gene expression studies demonstrate the presence of both undifferentiated cartilage and muscle precursors in mutants. However, chn blocks differentiation directly in neural crest, and not in mesoderm, as revealed by mosaic analyses. Neural crest cells taken from wild-type donor embryos can form cartilage when transplanted into chn mutant hosts and rescue some of the patterning defects of mutant pharyngeal arches. In these cases, cartilage only forms if neural crest is transplanted at least one hour before its migration, suggesting that interactions occur transiently in early jaw precursors. In contrast, transplanted cells in paraxial mesoderm behave according to the host genotype; mutant cells form jaw muscles in a wild-type environment. These results suggest that chn is required for the development of pharyngeal cartilages from cranial neural crest cells and subsequent crest signals that pattern mesodermally derived myocytes.
Genes / Markers
Marker Marker Type Name
chnGENEchinless
dlx2aGENEdistal-less homeobox 2a
1 - 2 of 2
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Figures
Figure Gallery (7 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
chnb146 (AB)standard conditionsPrim-15
chnb146 (AB)standard conditionsProtruding-mouth
chnb146 (AB)standard conditionsProtruding-mouth
chnb146 (AB)standard conditionsProtruding-mouth
chnb146 (AB)standard conditionsProtruding-mouth
chnb146 (AB)standard conditionsProtruding-mouth
chnb146 (AB)standard conditionsProtruding-mouth
chnb146 (AB)standard conditionsProtruding-mouth
chnb146 (AB)standard conditionsProtruding-mouth
chnb146 (AB)standard conditionsProtruding-mouth
1 - 10 of 19
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b146
    		Unknown
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    chnb146 (AB)
    WT
    1 - 2 of 2
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    Antibodies
    Orthology
    Engineered Foreign Genes
    No data available
    Mapping
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    Citation
    Schilling, T.F., Walker, C., and Kimmel, C.B. (1996) The chinless mutation and neural crest cell interactions in zebrafish jaw development. Development (Cambridge, England). 122:1417-1426.