PUBLICATION
Characterization of AluI repeats of zebrafish (Brachydanio rerio)
- Authors
- He, L., Zhu, Z., Faras, A.J., Guise, K.S., Hackett, P.B., and Kapuscinski, A.R.
- ID
- ZDB-PUB-961014-427
- Date
- 1992
- Source
- Molecular marine biology and biotechnology 1: 125-135 (Journal)
- Registered Authors
- Hackett, Perry B.
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- DNA/genetics*
- DNA/isolation & purification
- Deoxyribonucleases, Type II Site-Specific
- Genome
- Molecular Sequence Data
- Repetitive Sequences, Nucleic Acid*
- Restriction Mapping
- Sequence Deletion
- Sequence Homology, Nucleic Acid
- Zebrafish/genetics*
- PubMed
- 1308809
Citation
He, L., Zhu, Z., Faras, A.J., Guise, K.S., Hackett, P.B., and Kapuscinski, A.R. (1992) Characterization of AluI repeats of zebrafish (Brachydanio rerio). Molecular marine biology and biotechnology. 1:125-135.
Abstract
Two families of repetitive DNA sequences were isolated from the zebrafish genome and characterized. Eight different sequences were sequenced and classified by two standards, their (G + C) composition and their lengths. For convenience, the sequences were first divided into two types. Type I was (A + T)-rich, was repeated approximately 500,000 times, and constituted approximately 5% of the zebrafish genome. Type II was (G + C)-rich, was reiterated approximately 90,000 times, and comprised approximately 0.5% of the genome. Agarose gel electrophoresis of zebrafish DNA cleaved with AluI revealed three distinguishable bands of repetitive fragments: large (approximately 180 bp, designated RFAL), medium (approximately 140 bp, RFAM), and small (approximately 90 bp, RFAS). The RFAL fragments contained both type I and type II sequences. Limited digestion of genomic DNA indicated that RFAL and RFAM were tandemly arranged in the genome, whereas RFAS showed a mixed pattern of both tandem and interspersed repeated arrangements. Although inclusion of a repetitive sequence in a transgenic construct did not appreciably accelerate homologous integration of transgenes into the zebrafish genome, the AluI sequences could facilitate transgene mapping following chromosomal integration.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping