PUBLICATION
Fish gonadotropin-releasing hormone gene and molecular approaches for control of sexual maturation: development of a transgenic fish model
- Authors
- Aleström, P., Kisen, G., Klungland, H., and Andersen, O.
- ID
- ZDB-PUB-961014-25
- Date
- 1992
- Source
- Molecular marine biology and biotechnology 1: 376-379 (Journal)
- Registered Authors
- Aleström, Peter
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Animals, Genetically Modified
- Base Sequence
- DNA/isolation & purification
- Gene Expression Regulation
- Gonadotropin-Releasing Hormone/antagonists & inhibitors
- Gonadotropin-Releasing Hormone/genetics*
- Luciferases/biosynthesis
- Molecular Sequence Data
- Oryzias
- Protein Precursors/genetics
- Salmon/genetics
- Sequence Homology, Nucleic Acid
- Sexual Maturation/drug effects
- Sexual Maturation/physiology*
- Trout/genetics*
- Zebrafish
- PubMed
- 1308825
Citation
Aleström, P., Kisen, G., Klungland, H., and Andersen, O. (1992) Fish gonadotropin-releasing hormone gene and molecular approaches for control of sexual maturation: development of a transgenic fish model. Molecular marine biology and biotechnology. 1:376-379.
Abstract
The prepro-GnRH gene and mRNA primary structure were fully established from Atlantic salmon (Salmo salar) and partially from rainbow trout (Oncorhynchus mykiss). Results show that the GnRH coding region of 30 base pairs is well conserved during evolution. In contrast, the GnRH-associated peptide (GAP) sequence shows very limited homology when the GnRH genes from mammalian and teleost species are compared. A simple method for selecting transgenic fish after transfer of the firefly luciferase gene was developed. The method involves bioluminescent measurement of live animals in a scintillation counter.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping