PUBLICATION
Neural progenitor-derived Apelin controls tip cell behavior and vascular patterning
- Authors
- Malchow, J., Eberlein, J., Li, W., Hogan, B.M., Okuda, K.S., Helker, C.S.M.
- ID
- ZDB-PUB-240706-2
- Date
- 2024
- Source
- Science advances 10: eadk1174eadk1174 (Journal)
- Registered Authors
- Eberlein, Jean, Helker, Christian, Hogan, Ben M., Malchow, Julian, Okuda, Kazuhide Shaun
- Keywords
- none
- MeSH Terms
-
- Animals
- Apelin*/genetics
- Apelin*/metabolism
- Cell Movement
- Chemokines
- Intercellular Signaling Peptides and Proteins/genetics
- Intercellular Signaling Peptides and Proteins/metabolism
- Neovascularization, Physiologic*
- Neural Stem Cells*/cytology
- Neural Stem Cells*/metabolism
- Neural Tube/metabolism
- Phosphatidylinositol 3-Kinases/metabolism
- Signal Transduction*
- Zebrafish
- Zebrafish Proteins
- PubMed
- 38968355 Full text @ Sci Adv
Citation
Malchow, J., Eberlein, J., Li, W., Hogan, B.M., Okuda, K.S., Helker, C.S.M. (2024) Neural progenitor-derived Apelin controls tip cell behavior and vascular patterning. Science advances. 10:eadk1174eadk1174.
Abstract
During angiogenesis, vascular tip cells guide nascent vascular sprouts to form a vascular network. Apelin, an agonist of the G protein-coupled receptor Aplnr, is enriched in vascular tip cells, and it is hypothesized that vascular-derived Apelin regulates sprouting angiogenesis. We identify an apelin-expressing neural progenitor cell population in the dorsal neural tube. Vascular tip cells exhibit directed elongation and migration toward and along the apelin-expressing neural progenitor cells. Notably, restoration of neural but not vascular apelin expression in apelin mutants remedies the angiogenic defects of mutants. By functional analyses, we show the requirement of Apelin signaling for tip cell behaviors, like filopodia formation and cell elongation. Through genetic interaction studies and analysis of transgenic activity reporters, we identify Apelin signaling as a modulator of phosphoinositide 3-kinase and extracellular signal-regulated kinase signaling in tip cells in vivo. Our results suggest a previously unidentified neurovascular cross-talk mediated by Apelin signaling that is important for tip cell function during sprouting angiogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping