PUBLICATION
The evolutionarily conserved choroid plexus contributes to the homeostasis of brain ventricles in zebrafish
- Authors
- Jeong, I., Andreassen, S.N., Hoang, L., Poulain, M., Seo, Y., Park, H.C., Fürthauer, M., MacAulay, N., Jurisch-Yaksi, N.
- ID
- ZDB-PUB-240607-1
- Date
- 2024
- Source
- Cell Reports 43: 114331114331 (Journal)
- Registered Authors
- Fürthauer, Maximilian, Jurisch-Yaksi, Nathalie, Park, Hae-Chul
- Keywords
- CP: Neuroscience, brain ventricles, cerebrospinal fluid, choroid plexus, fabp7b, zebrafish
- MeSH Terms
-
- Animals
- Biological Evolution
- Blood-Brain Barrier/metabolism
- Cerebral Ventricles*/metabolism
- Cerebrospinal Fluid/metabolism
- Choroid Plexus*/metabolism
- Epithelial Cells/metabolism
- Homeostasis*
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 38843394 Full text @ Cell Rep.
Citation
Jeong, I., Andreassen, S.N., Hoang, L., Poulain, M., Seo, Y., Park, H.C., Fürthauer, M., MacAulay, N., Jurisch-Yaksi, N. (2024) The evolutionarily conserved choroid plexus contributes to the homeostasis of brain ventricles in zebrafish. Cell Reports. 43:114331114331.
Abstract
The choroid plexus (ChP) produces cerebrospinal fluid (CSF). It also contributes to brain development and serves as the CSF-blood barrier. Prior studies have identified transporters on the epithelial cells that transport water and ions from the blood vasculature to the ventricles and tight junctions involved in the CSF-blood barrier. Yet, how the ChP epithelial cells control brain physiology remains unresolved. We use zebrafish to provide insights into the physiological roles of the ChP. Upon histological and transcriptomic analyses, we identify that the zebrafish ChP is conserved with mammals and expresses transporters involved in CSF secretion. Next, we show that the ChP epithelial cells secrete proteins into CSF. By ablating the ChP epithelial cells, we identify a reduction of the ventricular sizes without alterations of the CSF-blood barrier. Altogether, our findings reveal that the zebrafish ChP is conserved and contributes to the size and homeostasis of the brain ventricles.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping