PUBLICATION
Cell-size-dependent regulation of Ezrin dictates epithelial resilience to stretch by countering myosin-II-mediated contractility
- Authors
- Chouhan, G., Lewis, N.S., Ghanekar, V., Koti Ainavarapu, S.R., Inamdar, M.M., Sonawane, M.
- ID
- ZDB-PUB-240602-11
- Date
- 2024
- Source
- Cell Reports 43: 114271114271 (Journal)
- Registered Authors
- Sonawane, Mahendra
- Keywords
- 2D vertex model, CP: Cell biology, CP: Developmental biology, Ezrin, cell adhesion, cell proliferation, cell size, epithelial resilience, epithelium, myosin contractility
- MeSH Terms
-
- Animals
- Cadherins/metabolism
- Cell Proliferation
- Cell Size*
- Cytoskeletal Proteins*/genetics
- Cytoskeletal Proteins*/metabolism
- Epidermis/metabolism
- Epithelial Cells/metabolism
- Epithelium/metabolism
- Myosin Type II*/metabolism
- Stress, Mechanical
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 38823013 Full text @ Cell Rep.
Citation
Chouhan, G., Lewis, N.S., Ghanekar, V., Koti Ainavarapu, S.R., Inamdar, M.M., Sonawane, M. (2024) Cell-size-dependent regulation of Ezrin dictates epithelial resilience to stretch by countering myosin-II-mediated contractility. Cell Reports. 43:114271114271.
Abstract
The epithelial adaptations to mechanical stress are facilitated by molecular and tissue-scale changes that include the strengthening of junctions, cytoskeletal reorganization, and cell-proliferation-mediated changes in tissue rheology. However, the role of cell size in controlling these properties remains underexplored. Our experiments in the zebrafish embryonic epidermis, guided by theoretical estimations, reveal a link between epithelial mechanics and cell size, demonstrating that an increase in cell size compromises the tissue fracture strength and compliance. We show that an increase in E-cadherin levels in the proliferation-deficient epidermis restores epidermal compliance but not the fracture strength, which is largely regulated by Ezrin-an apical membrane-cytoskeleton crosslinker. We show that Ezrin fortifies the epithelium in a cell-size-dependent manner by countering non-muscle myosin-II-mediated contractility. This work uncovers the importance of cell size maintenance in regulating the mechanical properties of the epithelium and fostering protection against future mechanical stresses.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping