PUBLICATION
The deletion of Asb15b gene can lead to a significant decrease in zebrafish intermuscular bone
- Authors
- Niu, M., Whang, H., Wu, Z., ShouwenJiang, ., Chen, L.
- ID
- ZDB-PUB-240517-7
- Date
- 2024
- Source
- Gene 923: 148561 (Journal)
- Registered Authors
- Chen, Liangbiao
- Keywords
- Asb15b gene, CRISPR/Cas9, Intermuscular bone, Zebrafish
- MeSH Terms
-
- Animals
- Bone Development/genetics
- Bone and Bones/metabolism
- CRISPR-Cas Systems
- Gene Deletion
- Gene Expression Regulation, Developmental
- Muscle, Skeletal/metabolism
- Zebrafish*/genetics
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 38754570 Full text @ Gene
Citation
Niu, M., Whang, H., Wu, Z., ShouwenJiang, ., Chen, L. (2024) The deletion of Asb15b gene can lead to a significant decrease in zebrafish intermuscular bone. Gene. 923:148561.
Abstract
Intercellular bones, which are present in numerous economically significant fish species, have a negative impact on the development of aquaculture. The Asb15b gene, primarily expressed in skeletal muscle, plays a crucial role in regulating protein turnover and the development of muscle fibers. It stimulates protein synthesis and controls the differentiation of muscle fibers. In this study, we employed CRISPR/Cas9 technology to generate homozygous zebrafish strains with 7 bp and 49 bp deletions in the Asb15b gene. Subsequent analyses using skeleton staining demonstrated a substantial reduction in the number of intermuscular bones in adult Asb15b-/- -7 bp and Asb15b-/- -49 bp mutants compared to the wild-type zebrafish, with decreases of 30 % (P < 0.001) and 40 % (P < 0.0001), respectively. Histological experiments further revealed that the diameter and number of muscle fibers in adult Asb15b-/- mutants did not exhibit significant changes when compared to wild-type zebrafish. Moreover, qRT-PCR experiments demonstrated significant differences in the expression of bmp6 and runx2b genes, which are key regulators of intermuscular bone development, during different stages of intermuscular bone development in Asb15b-/- mutants. This study strongly suggests that the Asb15b gene plays a crucial role in regulating intermuscular bone development in fish and lays the groundwork for further exploration of the role of the Asb15b gene in zebrafish intermuscular bone development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping