PUBLICATION
Upregulation of coagulation factor V by glucocorticoid in the preovulatory follicles of zebrafish
- Authors
- Huang, J., Sun, C., Huang, Z., Zhu, Y., Chen, S.X.
- ID
- ZDB-PUB-240418-6
- Date
- 2024
- Source
- The Journal of steroid biochemistry and molecular biology 241: 106521 (Journal)
- Registered Authors
- Zhu, Yong
- Keywords
- coagulation factor, glucocorticoid, glucocorticoid receptor, ovarian follicles
- MeSH Terms
-
- 11-beta-Hydroxysteroid Dehydrogenase Type 2/genetics
- 11-beta-Hydroxysteroid Dehydrogenase Type 2/metabolism
- Animals
- Female
- Glucocorticoids*/pharmacology
- Hydrocortisone*/metabolism
- Hydrocortisone*/pharmacology
- Ovarian Follicle*/drug effects
- Ovarian Follicle*/metabolism
- Ovulation/drug effects
- Promoter Regions, Genetic
- Receptors, Glucocorticoid*/genetics
- Receptors, Glucocorticoid*/metabolism
- Up-Regulation*/drug effects
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 38631601 Full text @ Steroid Biochem. Mol. Biol.
Citation
Huang, J., Sun, C., Huang, Z., Zhu, Y., Chen, S.X. (2024) Upregulation of coagulation factor V by glucocorticoid in the preovulatory follicles of zebrafish. The Journal of steroid biochemistry and molecular biology. 241:106521.
Abstract
Increased cortisol levels in the preovulatory follicular fluid suggests a role of glucocorticoid in human ovulation. However, the mechanisms through which cortisol regulates the ovulatory process remain poorly understood. In this study, we examined the upregulation of f5 mRNA by glucocorticoid and its receptor (Gr) in the preovulatory follicles of zebrafish. Our findings demonstrate a significant increase in 11β-hydroxysteroid dehydrogenase type 2 (hsd11b2), a cortisol response gene, in preovulatory follicles. Additionally, hydrocortisone exerts a dose- and time-dependent upregulation of f5 mRNA in these follicles. Importantly, this stimulatory effect is Gr-dependent, as it was completely abolished in gr-/- mutants. Furthermore, site-directed mutagenesis identified a glucocorticoid response element (GRE) in the promoter of zebrafish f5. Interestingly, successive incubation of hydrocortisone and the native ovulation-inducing steroid, progestin (17α,20β-dihydroxy-4-pregnen-3-one, DHP), further enhanced f5 expression in preovulatory follicles. Overall, our results indicate that the dramatic increase of f5 expression in preovulatory follicles is partially attributable to the regulation of glucocorticoid and Gr.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping