PUBLICATION
Differential Roles of Diet on Development and Spinal Cord Regeneration in Larval Zebrafish
- Authors
- Purifoy, E.J., Mruk, K.
- ID
- ZDB-PUB-240416-11
- Date
- 2024
- Source
- Zebrafish 21: 214222214-222 (Journal)
- Registered Authors
- Mruk, Karen
- Keywords
- behavior, cell biology, spinal cord injury, swimming, zebrafish
- MeSH Terms
-
- Animals
- Diet/veterinary
- Larva
- Reproducibility of Results
- Rotifera*
- Spinal Cord Regeneration*
- Zebrafish
- PubMed
- 38621204 Full text @ Zebrafish
Citation
Purifoy, E.J., Mruk, K. (2024) Differential Roles of Diet on Development and Spinal Cord Regeneration in Larval Zebrafish. Zebrafish. 21:214222214-222.
Abstract
The zebrafish is a powerful model organism for studying development and regeneration. However, there is a lack of a standardized reference diet for developmental and regeneration experiments. Most studies evaluate the rate of growth, survival, and fecundity. In this study, we compare three diets and their effects on growth and regeneration after a spinal cord injury (SCI). Fish were fed daily for 1 week with daily measurements of overall length and width of spinal injury. Fish fed a live rotifer diet grew 32%, whereas a commercially available diet only led to a 4% increase in body length. Similarly, differences in rate of regeneration were observed with over 80% of rotifer-fed larvae forming a glial bridge after injury compared to <10% of zebrafish fed with the commercial diet. Our data highlight the need for establishing a standardized diet for regeneration studies to improve research reproducibility.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping