PUBLICATION
Distinct features of the regenerating heart uncovered through comparative single-cell profiling
- Authors
- Carey, C.M., Hollins, H.L., Schmid, A.V., Gagnon, J.A.
- ID
- ZDB-PUB-240326-2
- Date
- 2024
- Source
- Biology Open 13(4): (Journal)
- Registered Authors
- Carey, Clayton, Gagnon, James, Schmid, Alexis
- Keywords
- Evolution, Heart, Immunity, Interferon, Myocardium, Regeneration
- MeSH Terms
-
- Adult
- Animals
- Biological Evolution
- Humans
- Mammals
- Myocardium*/metabolism
- Myocytes, Cardiac/metabolism
- Zebrafish*/genetics
- PubMed
- 38526188 Full text @ Biol. Open
Citation
Carey, C.M., Hollins, H.L., Schmid, A.V., Gagnon, J.A. (2024) Distinct features of the regenerating heart uncovered through comparative single-cell profiling. Biology Open. 13(4):.
Abstract
Adult humans respond to heart injury by forming a permanent scar, yet other vertebrates are capable of robust and complete cardiac regeneration. Despite progress towards characterizing the mechanisms of cardiac regeneration in fish and amphibians, the large evolutionary gulf between mammals and regenerating vertebrates complicates deciphering which cellular and molecular features truly enable regeneration. To better define these features, we compared cardiac injury responses in zebrafish and medaka, two fish species that share similar heart anatomy and common teleost ancestry but differ in regenerative capability. We used single-cell transcriptional profiling to create a time-resolved comparative cell atlas of injury responses in all major cardiac cell types across both species. With this approach, we identified several key features that distinguish cardiac injury response in the non-regenerating medaka heart. By comparing immune responses to injury, we found altered cell recruitment and a distinct pro-inflammatory gene program in medaka leukocytes, and an absence of the injury-induced interferon response seen in zebrafish. In addition, we found a lack of pro-regenerative signals, including nrg1 and retinoic acid, from medaka endothelial and epicardial cells. Finally, we identified alterations in the myocardial structure in medaka, where they lack primordial layer cardiomyocytes and fail to employ a cardioprotective gene program shared by regenerating vertebrates. Our findings reveal notable variation in injury response across nearly all major cardiac cell types in zebrafish and medaka, demonstrating how evolutionary divergence influences the hidden cellular features underpinning regenerative potential in these seemingly similar vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping