PUBLICATION
The forkhead transcription factor Foxj1 controls vertebrate olfactory cilia biogenesis and sensory neuron differentiation
- Authors
- Rayamajhi, D., Ege, M., Ukhanov, K., Ringers, C., Zhang, Y., Jung, I., D'Gama, P.P., Li, S.S., Cosacak, M.I., Kizil, C., Park, H.C., Yaksi, E., Martens, J.R., Brody, S.L., Jurisch-Yaksi, N., Roy, S.
- ID
- ZDB-PUB-240126-5
- Date
- 2024
- Source
- PLoS Biology 22: e3002468e3002468 (Journal)
- Registered Authors
- Cosacak, Mehmet Ilyas, Jurisch-Yaksi, Nathalie, Kizil, Caghan, Park, Hae-Chul, Ringers, Christa, Roy, Sudipto, Yaksi, Emre
- Keywords
- none
- Datasets
- GEO:GSE232397
- MeSH Terms
-
- Animals
- Cilia/metabolism
- Forkhead Transcription Factors/genetics
- Forkhead Transcription Factors/metabolism
- Humans
- Mice
- Olfactory Mucosa
- Olfactory Receptor Neurons*
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 38271330 Full text @ PLoS Biol.
Citation
Rayamajhi, D., Ege, M., Ukhanov, K., Ringers, C., Zhang, Y., Jung, I., D'Gama, P.P., Li, S.S., Cosacak, M.I., Kizil, C., Park, H.C., Yaksi, E., Martens, J.R., Brody, S.L., Jurisch-Yaksi, N., Roy, S. (2024) The forkhead transcription factor Foxj1 controls vertebrate olfactory cilia biogenesis and sensory neuron differentiation. PLoS Biology. 22:e3002468e3002468.
Abstract
In vertebrates, olfactory receptors localize on multiple cilia elaborated on dendritic knobs of olfactory sensory neurons (OSNs). Although olfactory cilia dysfunction can cause anosmia, how their differentiation is programmed at the transcriptional level has remained largely unexplored. We discovered in zebrafish and mice that Foxj1, a forkhead domain-containing transcription factor traditionally linked with motile cilia biogenesis, is expressed in OSNs and required for olfactory epithelium (OE) formation. In keeping with the immotile nature of olfactory cilia, we observed that ciliary motility genes are repressed in zebrafish, mouse, and human OSNs. Strikingly, we also found that besides ciliogenesis, Foxj1 controls the differentiation of the OSNs themselves by regulating their cell type-specific gene expression, such as that of olfactory marker protein (omp) involved in odor-evoked signal transduction. In line with this, response to bile acids, odors detected by OMP-positive OSNs, was significantly diminished in foxj1 mutant zebrafish. Taken together, our findings establish how the canonical Foxj1-mediated motile ciliogenic transcriptional program has been repurposed for the biogenesis of immotile olfactory cilia, as well as for the development of the OSNs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping