PUBLICATION
HB-EGF promotes progenitor cell proliferation and sensory neuron regeneration in the zebrafish olfactory epithelium
- Authors
- Sireci, S., Kocagöz, Y., Alkiraz, A.S., Güler, K., Dokuzluoglu, Z., Balcioglu, E., Meydanli, S., Demirler, M.C., Sögünmez Erdogan, N., Fuss, S.H.
- ID
- ZDB-PUB-231213-12
- Date
- 2023
- Source
- The FEBS journal 291(10): 2098-2133 (Journal)
- Registered Authors
- Fuss, Stefan
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Proliferation*/drug effects
- ErbB Receptors*/genetics
- ErbB Receptors*/metabolism
- Heparin-binding EGF-like Growth Factor*/genetics
- Heparin-binding EGF-like Growth Factor*/metabolism
- Intercellular Signaling Peptides and Proteins/genetics
- Intercellular Signaling Peptides and Proteins/metabolism
- Intercellular Signaling Peptides and Proteins/pharmacology
- Nerve Regeneration/drug effects
- Neurogenesis/drug effects
- Olfactory Mucosa*/cytology
- Olfactory Mucosa*/drug effects
- Olfactory Mucosa*/metabolism
- Olfactory Receptor Neurons/cytology
- Olfactory Receptor Neurons/drug effects
- Olfactory Receptor Neurons/metabolism
- Signal Transduction
- Stem Cells/cytology
- Stem Cells/drug effects
- Stem Cells/metabolism
- Zebrafish*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 38088047 Full text @ FEBS J.
Citation
Sireci, S., Kocagöz, Y., Alkiraz, A.S., Güler, K., Dokuzluoglu, Z., Balcioglu, E., Meydanli, S., Demirler, M.C., Sögünmez Erdogan, N., Fuss, S.H. (2023) HB-EGF promotes progenitor cell proliferation and sensory neuron regeneration in the zebrafish olfactory epithelium. The FEBS journal. 291(10):2098-2133.
Abstract
Maintenance and regeneration of the zebrafish olfactory epithelium (OE) are supported by two distinct progenitor cell populations that occupy spatially discrete stem cell niches and respond to different tissue conditions. Globose basal cells (GBCs) reside at the inner and peripheral margins of the sensory OE and are constitutively active to replace sporadically dying olfactory sensory neurons (OSNs). In contrast, horizontal basal cells (HBCs) are uniformly distributed across the sensory tissue and are selectively activated by acute injury conditions. Here we show that expression of the heparin-binding epidermal growth factor-like growth factor (HB-EGF) is strongly and transiently upregulated in response to OE injury and signals through the EGF receptor (EGFR), which is expressed by HBCs. Exogenous stimulation of the OE with recombinant HB-EGF promotes HBC expansion and OSN neurogenesis in a pattern that resembles the tissue response to injury. In contrast, pharmacological inhibition of HB-EGF membrane shedding, HB-EGF availability, and EGFR signaling strongly attenuate or delay injury-induced HBC activity and OSN restoration without affecting maintenance neurogenesis by GBCs. Thus, HB-EGF/EGFR signaling appears to be a critical component of the signaling network that controls HBC activity and, consequently, repair neurogenesis in the zebrafish OE.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping