PUBLICATION
Rationally Designed Self-Assembling Nanovaccines Elicit Robust Mucosal and Systemic Immunity against Rhabdovirus
- Authors
- Zhang, C., Zhao, Z., Jia, Y.J., Zhang, P.Q., Sun, Y., Zhou, Y.C., Wang, G.X., Zhu, B.
- ID
- ZDB-PUB-231207-4
- Date
- 2023
- Source
- ACS applied materials & interfaces 16(1): 228-244 (Journal)
- Registered Authors
- Keywords
- adaptive immune, antigen screening, apoferritin-based nanovaccine, immune mechanism, rhabdovirus, toll-like receptor 2
- MeSH Terms
-
- Animals
- Nanoparticles*/chemistry
- Nanovaccines
- Rhabdoviridae*
- Toll-Like Receptor 2
- Vaccines*
- Zebrafish
- PubMed
- 38055273 Full text @ ACS Appl. Mater. Interfaces
Citation
Zhang, C., Zhao, Z., Jia, Y.J., Zhang, P.Q., Sun, Y., Zhou, Y.C., Wang, G.X., Zhu, B. (2023) Rationally Designed Self-Assembling Nanovaccines Elicit Robust Mucosal and Systemic Immunity against Rhabdovirus. ACS applied materials & interfaces. 16(1):228-244.
Abstract
Viral diseases have constantly caused great threats to global public health, resulting in an urgent need for effective vaccines. However, the current viral vaccines often show low immunogenicity. To counter this, we report a smart strategy of a well-designed modular nanoparticle (LSG-TDH) that recapitulates the dominant antigen SG, low-molecular-weight protamine, and tetralysine-modified H-chain apoferritin (TDH). The constructed LSG-TDH nanovaccine could self-assemble into a nanocage structure, which confers excellent mucus-penetrating, cellular affinity, and uptake ability. Studies demonstrate that the LSG-TDH nanovaccine could strongly activate both mucosal and systemic immune responses. Importantly, by immunizing wild-type and TLR2 knockout (TLR2-KO) zebrafish, we found that TLR2 could mediate LSG-TDH-induced adaptive mucosal and systemic immune responses by activating antigen-presenting cells. Collectively, our findings offer new insights into rational viral vaccine design and provide additional evidence of the vital role of TLR2 in regulating adaptive immunity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping