PUBLICATION
Hemogenic and aortic endothelium arise from a common hemogenic angioblast precursor and are specified by the Etv2 dosage
- Authors
- Zhao, S., Feng, S., Tian, Y., Wen, Z.
- ID
- ZDB-PUB-231130-25
- Date
- 2022
- Source
- Proceedings of the National Academy of Sciences of the United States of America 119: e2119051119e2119051119 (Journal)
- Registered Authors
- Wen, Zilong
- Keywords
- endothelium, fate determination, hemogenic endothelium, lineage origin
- Datasets
- GEO:GSE197757
- MeSH Terms
-
- Animals
- Cell Differentiation
- Endothelium, Vascular
- Hemangioblasts*
- Hematopoiesis*
- PubMed
- 35333649 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Zhao, S., Feng, S., Tian, Y., Wen, Z. (2022) Hemogenic and aortic endothelium arise from a common hemogenic angioblast precursor and are specified by the Etv2 dosage. Proceedings of the National Academy of Sciences of the United States of America. 119:e2119051119e2119051119.
Abstract
SignificanceHematopoietic stem cells (HSCs) are generated from specialized endothelial cells, called hemogenic endothelial cells (HECs). It has been debated whether HECs and non-HSC-forming conventional endothelial cells (cECs) arise from a common precursor or represent distinct lineages. Moreover, the molecular basis underlying their distinct fate determination is poorly understood. We use photoconvertible labeling, time-lapse imaging, and single-cell RNA-sequencing analysis to trace the lineage of HECs. We discovered that HECs and cECs arise from a common hemogenic angioblast precursor, and their distinct fate is determined by high or low dosage of Etv2, respectively. Our results illuminate the lineage origin and a mechanism on the fate determination of HECs, which may enhance the understanding on the ontogeny of HECs in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping