PUBLICATION
Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB
- Authors
- Zou, J., Anai, S., Ota, S., Ishitani, S., Oginuma, M., Ishitani, T.
- ID
- ZDB-PUB-231109-3
- Date
- 2023
- Source
- Nature communications 14: 71947194 (Journal)
- Registered Authors
- Ishitani, Shizuka, Ishitani, Tohru, Oginuma, Masayuki, Zou, Juqi
- Keywords
- none
- MeSH Terms
-
- Animals
- Drosophila
- Feedback
- Ligands
- NF-kappa B*
- Wnt Signaling Pathway
- Zebrafish*
- PubMed
- 37938219 Full text @ Nat. Commun.
Citation
Zou, J., Anai, S., Ota, S., Ishitani, S., Oginuma, M., Ishitani, T. (2023) Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB. Nature communications. 14:71947194.
Abstract
In vertebrate embryos, the canonical Wnt ligand primes the formation of dorsal organizers that govern dorsal-ventral patterns by secreting BMP antagonists. In contrast, in Drosophila embryos, Toll-like receptor (Tlr)-mediated NFκB activation initiates dorsal-ventral patterning, wherein Wnt-mediated negative feedback regulation of Tlr/NFκB generates a BMP antagonist-secreting signalling centre to control the dorsal-ventral pattern. Although both Wnt and BMP antagonist are conserved among species, the involvement of Tlr/NFκB and feedback regulation in vertebrate organizer formation remains unclear. By imaging and genetic modification, we reveal that a negative feedback loop between canonical and non-canonical Wnts and Tlr4/NFκB determines the size of zebrafish organizer, and that Tlr/NFκB and Wnts switch initial cue and feedback mediator roles between Drosophila and zebrafish. Here, we show that canonical Wnt signalling stimulates the expression of the non-canonical Wnt5b ligand, activating the Tlr4 receptor to stimulate NFκB-mediated transcription of the Wnt antagonist frzb, restricting Wnt-dependent dorsal organizer formation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping