PUBLICATION
De novo missense variants in phosphatidylinositol kinase PIP5KIγ underlie a neurodevelopmental syndrome associated with altered phosphoinositide signaling
- Authors
- Morleo, M., Venditti, R., Theodorou, E., Briere, L.C., Rosello, M., Tirozzi, A., Tammaro, R., Al-Badri, N., High, F.A., Shi, J., Undiagnosed Diseases Network, Telethon Undiagnosed Diseases Program, Putti, E., Ferrante, L., Cetrangolo, V., Torella, A., Walker, M.A., Tenconi, R., Iascone, M., Mei, D., Guerrini, R., van der Smagt, J., Kroes, H.Y., van Gassen, K.L.I., Bilal, M., Umair, M., Pingault, V., Attie-Bitach, T., Amiel, J., Ejaz, R., Rodan, L., Zollino, M., Agrawal, P.B., Del Bene, F., Nigro, V., Sweetser, D.A., Franco, B.
- ID
- ZDB-PUB-230716-37
- Date
- 2023
- Source
- American journal of human genetics 110(8): 1377-1393 (Journal)
- Registered Authors
- Del Bene, Filippo, Rosello, Marion
- Keywords
- PIP5K1C, de novo gain-of-function variants, developmental delay, endosomes, intellectual disability, phosphatidylinositol 4,5 bisphosphate (PI(4,5)P(2)), phosphoinositides, zebrafish
- MeSH Terms
-
- Actins
- Animals
- Intellectual Disability*/genetics
- Phosphatidylinositol Phosphates
- Phosphatidylinositols*
- Phosphoric Monoester Hydrolases/genetics
- Syndrome
- Zebrafish/genetics
- PubMed
- 37451268 Full text @ Am. J. Hum. Genet.
Citation
Morleo, M., Venditti, R., Theodorou, E., Briere, L.C., Rosello, M., Tirozzi, A., Tammaro, R., Al-Badri, N., High, F.A., Shi, J., Undiagnosed Diseases Network, Telethon Undiagnosed Diseases Program, Putti, E., Ferrante, L., Cetrangolo, V., Torella, A., Walker, M.A., Tenconi, R., Iascone, M., Mei, D., Guerrini, R., van der Smagt, J., Kroes, H.Y., van Gassen, K.L.I., Bilal, M., Umair, M., Pingault, V., Attie-Bitach, T., Amiel, J., Ejaz, R., Rodan, L., Zollino, M., Agrawal, P.B., Del Bene, F., Nigro, V., Sweetser, D.A., Franco, B. (2023) De novo missense variants in phosphatidylinositol kinase PIP5KIγ underlie a neurodevelopmental syndrome associated with altered phosphoinositide signaling. American journal of human genetics. 110(8):1377-1393.
Abstract
Phosphoinositides (PIs) are membrane phospholipids produced through the local activity of PI kinases and phosphatases that selectively add or remove phosphate groups from the inositol head group. PIs control membrane composition and play key roles in many cellular processes including actin dynamics, endosomal trafficking, autophagy, and nuclear functions. Mutations in phosphatidylinositol 4,5 bisphosphate [PI(4,5)P2] phosphatases cause a broad spectrum of neurodevelopmental disorders such as Lowe and Joubert syndromes and congenital muscular dystrophy with cataracts and intellectual disability, which are thus associated with increased levels of PI(4,5)P2. Here, we describe a neurodevelopmental disorder associated with an increase in the production of PI(4,5)P2 and with PI-signaling dysfunction. We identified three de novo heterozygous missense variants in PIP5K1C, which encodes an isoform of the phosphatidylinositol 4-phosphate 5-kinase (PIP5KIγ), in nine unrelated children exhibiting intellectual disability, developmental delay, acquired microcephaly, seizures, visual abnormalities, and dysmorphic features. We provide evidence that the PIP5K1C variants result in an increase of the endosomal PI(4,5)P2 pool, giving rise to ectopic recruitment of filamentous actin at early endosomes (EEs) that in turn causes dysfunction in EE trafficking. In addition, we generated an in vivo zebrafish model that recapitulates the disorder we describe with developmental defects affecting the forebrain, including the eyes, as well as craniofacial abnormalities, further demonstrating the pathogenic effect of the PIP5K1C variants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping