PUBLICATION
Erk5 functions in modulation of zebrafish intestinal permeability
- Authors
- Lv, H., Jin, Z., Wang, D., Guo, X., Wang, H., Yang, S.
- ID
- ZDB-PUB-230601-34
- Date
- 2023
- Source
- Cell and tissue research 393(2): 281-296 (Journal)
- Registered Authors
- Yang, Shulan
- Keywords
- Erk5 knockout, Intestinal morphogenesis, Intestinal permeability, Zebrafish
- MeSH Terms
-
- Animals
- Epithelial Cells/metabolism
- Intestinal Mucosa/metabolism
- Intestines
- Mitogen-Activated Protein Kinase 7*
- Permeability
- Zebrafish*/metabolism
- PubMed
- 37256363 Full text @ Cell Tissue Res.
Citation
Lv, H., Jin, Z., Wang, D., Guo, X., Wang, H., Yang, S. (2023) Erk5 functions in modulation of zebrafish intestinal permeability. Cell and tissue research. 393(2):281-296.
Abstract
The intestine of zebrafish consists of mucosa, muscularis and serosa. Intestinal epithelial cells (IECs) act as a physical and biochemical barrier to protect against invasion by external commensal bacteria. Cell junction is one of the crucial basis of the barrier function. When cell junctions were disrupted, intestinal permeability would be naturally impeded. Extracellular signal-regulated kinase 5 (ERK5), belonging to the Mitogen-activated protein kinase (MAPK) family, is involved in the normal physiological development of the cardiovascular system and nervous system. But the role of erk5 in intestinal morphogenesis and intestinal function is yet to know. Here, we showed that knockout of the erk5 in zebrafish larvae resulted in intestinal wall hypoplasia, including the thinned intestinal wall, reduced intestinal folds, and disrupted cell junctions. In addition, the intestinal permeability assay demonstrated that knockout of erk5 resulted in increased intestinal permeability. All of these showed that erk5 plays an essential role in the maintenance of intestinal barrier function. Thus, our data indicate that erk5 is a critical effector in intestinal morphogenesis and intestinal function, and dysfunction of erk5 would lead to intestinal diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping