PUBLICATION
"Lighting up" fluoride: cellular imaging and zebrafish model interrogations using a simple ESIPT-based mycophenolic acid precursor-based probe
- Authors
- Jain, N., Sonawane, P.M., Liu, H., Roychaudhury, A., Lee, Y., An, J., Kim, D., Kim, D., Kim, Y., Kim, Y.C., Cho, K.B., Park, H.S., Kim, C.H., Churchill, D.G.
- ID
- ZDB-PUB-230516-53
- Date
- 2023
- Source
- The Analyst 148(11): 2609-2615 (Journal)
- Registered Authors
- Kim, Cheol-Hee
- Keywords
- none
- MeSH Terms
-
- Animals
- Fluorescent Dyes
- Fluorides*
- HEK293 Cells
- Humans
- Mycophenolic Acid
- Zebrafish*
- PubMed
- 37190984 Full text @ Analyst
Citation
Jain, N., Sonawane, P.M., Liu, H., Roychaudhury, A., Lee, Y., An, J., Kim, D., Kim, D., Kim, Y., Kim, Y.C., Cho, K.B., Park, H.S., Kim, C.H., Churchill, D.G. (2023) "Lighting up" fluoride: cellular imaging and zebrafish model interrogations using a simple ESIPT-based mycophenolic acid precursor-based probe. The Analyst. 148(11):2609-2615.
Abstract
The discovery and implementation of media that derive from bioinspired designs and bear optical readouts featuring large Stokes shifts are of continued interest to a wide variety of researchers and clinicians. Myco-F, a novel mycophenolic acid precursor-based probe features a cleavable tert-butyldimethylsiloxy group to allow for fluoride detection. Myco-F exhibits high selectivity and specificity towards F- (Stokes shift = 120 nm). All measurements were performed in complete aqueous media (LOD=0.38 μM). Myco-F enables detection of fluoride ions in living HEK293 cells and localizes in the eye region (among other regions) of the zebrafish. DFT calculations support the proposed ESIPT working photomechanism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping