PUBLICATION
Transcriptomics and Phenotypic Analysis of gpr56 Knockout in Zebrafish
- Authors
- Sun, L., Yang, B., Peng, Z., Yang, T., Qin, B., Ao, J., Yang, Y., Wang, J., Zheng, L., Xie, H.
- ID
- ZDB-PUB-230514-35
- Date
- 2023
- Source
- International Journal of Molecular Sciences 24(9): (Journal)
- Registered Authors
- Xie, Huaping
- Keywords
- RNA-seq, differentially expressed genes, gpr56, innate immunity, knockout, motor ability, pancreas, zebrafish
- MeSH Terms
-
- Animals
- Humans
- Mutation
- Receptors, G-Protein-Coupled/metabolism
- Transcriptome*
- PubMed
- 37175447 Full text @ Int. J. Mol. Sci.
Abstract
The adhesion G-protein-coupled receptor is a seven-transmembrane receptor protein with a complex structure. Impaired GPR56 has been found to cause developmental damage to the human brain, resulting in intellectual disability and motor dysfunction. To date, studies on gpr56 deficiency in zebrafish have been limited to the nervous system, and there have been no reports of its systemic effects on juvenile fish at developmental stages. In order to explore the function of gpr56 in zebrafish, the CRISPR/Cas9 gene-editing system was used to construct a gpr56-knockout zebrafish. Subsequently, the differentially expressed genes (DEGs) at the transcriptional level between the 3 days post fertilization (dpf) homozygotes of the gpr56 mutation and the wildtype zebrafish were analyzed via RNA-seq. The results of the clustering analysis, quantitative PCR (qPCR), and in situ hybridization demonstrated that the expression of innate immunity-related genes in the mutant was disordered, and multiple genes encoding digestive enzymes of the pancreatic exocrine glands were significantly downregulated in the mutant. Motor ability tests demonstrated that the gpr56-/- zebrafish were more active, and this change was more pronounced in the presence of cold and additional stimuli. In conclusion, our results revealed the effect of gpr56 deletion on the gene expression of juvenile zebrafish and found that the gpr56 mutant was extremely active, providing an important clue for studying the mechanism of gpr56 in the development of juvenile zebrafish.
Genes / Markers
Figure Gallery (6 images)
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping