PUBLICATION
Actin filaments accumulated in the nucleus and remain in the vicinity of condensing chromosomes during prophase in zebrafish early embryo
- Authors
- Oda, H., Sato, Y., Kawashima, S.A., Fujiwara, Y., Pálfy, M., Wu, E., Vastenhouw, N.L., Kanai, M., Kimura, H.
- ID
- ZDB-PUB-230420-57
- Date
- 2023
- Source
- Biology Open 12(5): (Journal)
- Registered Authors
- Vastenhouw, Nadine
- Keywords
- Live-cell imaging, Nuclear actin, Zebrafish embryo
- MeSH Terms
-
- Actin Cytoskeleton
- Actins*
- Animals
- Chromosomes/genetics
- Mitosis
- Zebrafish*
- PubMed
- 37071022 Full text @ Biol. Open
Citation
Oda, H., Sato, Y., Kawashima, S.A., Fujiwara, Y., Pálfy, M., Wu, E., Vastenhouw, N.L., Kanai, M., Kimura, H. (2023) Actin filaments accumulated in the nucleus and remain in the vicinity of condensing chromosomes during prophase in zebrafish early embryo. Biology Open. 12(5):.
Abstract
In the cytoplasm, filamentous actin (F-actin) plays a critical role in cell regulation, including cell migration, stress fiber formation, and cytokinesis. Recent studies have shown that actin filaments that form in the nucleus are associated with diverse functions. Here, using live imaging of an F-actin-specific probe, superfolder GFP-tagged utrophin (UtrCH-sfGFP), we demonstrated the dynamics of nuclear actin in zebrafish (Danio rerio) embryos. In early zebrafish embryos up to around the high stage, UtrCH-sfGFP increasingly accumulated in nuclei during the interphase and reached a peak during the prophase. After nuclear envelope breakdown (NEBD), patches of UtrCH-sfGFP remained in the vicinity of condensing chromosomes during the prometaphase to metaphase. When zygotic transcription was inhibited by injecting α-amanitin, the nuclear accumulation of UtrCH-sfGFP was still observed at the sphere and dome stages, suggesting that zygotic transcription may induce a decrease in nuclear F-actin. The accumulation of F-actin in nuclei may contribute to proper mitotic progression of large cells with rapid cell cycles in zebrafish early embryos, by assisting in NEBD, chromosome congression, and/or spindle assembly.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping