PUBLICATION
Comprehensive maturity of nuclear pore complexes regulates zygotic genome activation
- Authors
- Shen, W., Gong, B., Xing, C., Zhang, L., Sun, J., Chen, Y., Yang, C., Yan, L., Chen, L., Yao, L., Li, G., Deng, H., Wu, X., Meng, A.
- ID
- ZDB-PUB-221210-10
- Date
- 2022
- Source
- Cell 185(26): 4954-4970.e20 (Journal)
- Registered Authors
- Gong, Bo, Meng, Anming, Xing, Cencan
- Keywords
- embryo, midblastula transition, nuclear pore complex, nuclei, transcription factor, zebrafish, zygotic genome activation
- Datasets
- GEO:GSE212929, GEO:GSE212933
- MeSH Terms
-
- Animals
- Embryonic Development/genetics
- Gene Expression Regulation, Developmental
- Genome
- Nuclear Pore*/metabolism
- Nuclear Pore Complex Proteins/genetics
- Nuclear Pore Complex Proteins/metabolism
- Transcription Factors/metabolism
- Zebrafish*/metabolism
- Zygote/metabolism
- PubMed
- 36493774 Full text @ Cell
Citation
Shen, W., Gong, B., Xing, C., Zhang, L., Sun, J., Chen, Y., Yang, C., Yan, L., Chen, L., Yao, L., Li, G., Deng, H., Wu, X., Meng, A. (2022) Comprehensive maturity of nuclear pore complexes regulates zygotic genome activation. Cell. 185(26):4954-4970.e20.
Abstract
Nuclear pore complexes (NPCs) are channels for nucleocytoplasmic transport of proteins and RNAs. However, it remains unclear whether composition, structure, and permeability of NPCs dynamically change during the cleavage period of vertebrate embryos and affect embryonic development. Here, we report that the comprehensive NPC maturity (CNM) controls the onset of zygotic genome activation (ZGA) during zebrafish early embryogenesis. We show that more nucleoporin proteins are recruited to and assembled into NPCs with development, resulting in progressive increase of NPCs in size and complexity. Maternal transcription factors (TFs) transport into nuclei more efficiently with increasing CNM. Deficiency or dysfunction of Nup133 or Ahctf1/Elys impairs NPC assembly, maternal TFs nuclear transport, and ZGA onset, while nup133 overexpression promotes these processes. Therefore, CNM may act as a molecular timer for ZGA by controlling nuclear transport of maternal TFs that reach nuclear concentration thresholds at a given time to initiate ZGA.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping