PUBLICATION
The calcium-sensing receptor (CaSR) regulates zebrafish sensorimotor decision making via a genetically defined cluster of hindbrain neurons
- Authors
- Shoenhard, H., Jain, R.A., Granato, M.
- ID
- ZDB-PUB-221209-5
- Date
- 2022
- Source
- Cell Reports 41: 111790111790 (Journal)
- Registered Authors
- Granato, Michael, Jain, Roshan
- Keywords
- CP: Neuroscience, CaSR, LLC, Mauthner, SLC, behavioral choice, circuit, decision making, neurogenetics, sensorimotor, startle response
- MeSH Terms
-
- Animals
- Receptors, Calcium-Sensing*/genetics
- Zebrafish*/genetics
- PubMed
- 36476852 Full text @ Cell Rep.
Citation
Shoenhard, H., Jain, R.A., Granato, M. (2022) The calcium-sensing receptor (CaSR) regulates zebrafish sensorimotor decision making via a genetically defined cluster of hindbrain neurons. Cell Reports. 41:111790111790.
Abstract
Decision making is a fundamental nervous system function that ranges widely in complexity and speed of execution. We previously established larval zebrafish as a model for sensorimotor decision making and identified the G-protein-coupled calcium-sensing receptor (CaSR) to be critical for this process. Here, we report that CaSR functions in neurons to dynamically regulate the bias between two behavioral outcomes: escapes and reorientations. By employing a computational guided transgenic strategy, we identify a genetically defined neuronal cluster in the hindbrain as a key candidate site for CaSR function. Finally, we demonstrate that transgenic CaSR expression targeting this cluster consisting of a few hundred neurons shifts behavioral bias in wild-type animals and restores decision making deficits in CaSR mutants. Combined, our data provide a rare example of a G-protein-coupled receptor that biases vertebrate sensorimotor decision making via a defined neuronal cluster.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping