PUBLICATION
Foxl2a and Foxl2b are involved in midbrain-hindbrain boundary development in zebrafish
- Authors
- Zhou, J., Yang, Y.J., Gan, R.H., Wang, Y., Li, Z., Zhang, X.J., Gui, J.F., Zhou, L.
- ID
- ZDB-PUB-221108-13
- Date
- 2022
- Source
- Gene expression patterns : GEP 46: 119286 (Journal)
- Registered Authors
- Gui, Jian-Fang, Li, Zhi, Wang, Yang, Zhou, Li
- Keywords
- Brain ventricular system, Embryogenesis, Midbrain-hindbrain boundary, Zebrafish, foxl2
- MeSH Terms
-
- Animals
- Embryonic Development
- Gene Expression Regulation, Developmental
- Mesencephalon/metabolism
- Rhombencephalon
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 36341978 Full text @ Gene Expr. Patterns
Citation
Zhou, J., Yang, Y.J., Gan, R.H., Wang, Y., Li, Z., Zhang, X.J., Gui, J.F., Zhou, L. (2022) Foxl2a and Foxl2b are involved in midbrain-hindbrain boundary development in zebrafish. Gene expression patterns : GEP. 46:119286.
Abstract
Foxl2 plays conserved central function in ovarian differentiation and maintenance in several fish species. However, its expression pattern and function in fish embryogenesis are still largely unknown. In this study, we first presented a sequential expression pattern of zebrafish foxl2a and foxl2b during embryo development. They were predominantly expressed in the cranial paraxial mesoderm (CPM) and cranial venous vasculature (CVV) during somitogenesis and subsequently expressed in the pharyngeal arches after 48 h post-fertilization (hpf). Then, we compared the brain structures among zebrafish wildtype (WT) and three homozygous foxl2 mutants (foxl2a-/-, foxl2b-/- and foxl2a-/-;foxl2b-/-) and found the reduction of the fourth ventricle in the three foxl2 mutants, especially in foxl2a-/-;foxl2b-/- mutant. Finally, we detected several key transcription factors involved in the gene regulatory network of midbrain-hindbrain boundary (MHB) patterning, such as wnt1, en1b and pax2a. Their expression levels were obviously downregulated in MHB of foxl2a-/- and foxl2a-/-;foxl2b-/- mutants. Thus, we suggest that Foxl2a and Foxl2b are involved in MHB and the fourth ventricle development in zebrafish. The current study provides insights into the molecular mechanism underlying development of brain ventricular system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping