PUBLICATION
Gsx2, but not Gsx1, is necessary for early forebrain patterning and long-term survival in zebrafish
- Authors
- Coltogirone, R.A., Sherfinski, E.I., Dobler, Z.A., Peterson, S.N., Andlinger, A.R., Fadel, L.C., Patrick, R.L., Bergeron, S.A.
- ID
- ZDB-PUB-221004-5
- Date
- 2022
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 252(3): 377-399 (Journal)
- Registered Authors
- Bergeron, Sadie, Patrick, Regina
- Keywords
- forebrain patterning, gs homeobox, neurodevelopment, transcription factor, zebrafish
- MeSH Terms
-
- Animals
- Gene Expression Regulation, Developmental
- Homeodomain Proteins*/genetics
- Olfactory Bulb/metabolism
- Telencephalon/metabolism
- Transcription Factors/metabolism
- Zebrafish*/genetics
- Zebrafish Proteins/genetics
- PubMed
- 36184733 Full text @ Dev. Dyn.
Citation
Coltogirone, R.A., Sherfinski, E.I., Dobler, Z.A., Peterson, S.N., Andlinger, A.R., Fadel, L.C., Patrick, R.L., Bergeron, S.A. (2022) Gsx2, but not Gsx1, is necessary for early forebrain patterning and long-term survival in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 252(3):377-399.
Abstract
Background Homeobox transcription factor encoding genes, genomic screen homeobox 1 and 2 (gsx1 and gsx2), are expressed during neurodevelopment in multiple vertebrates. However, we have limited knowledge of the dynamic expression of these genes through developmental time and the gene networks that they regulate in zebrafish.
Results We confirmed that gsx1 is expressed initially in the hindbrain and diencephalon and later in the optic tectum, pretectum, and cerebellar plate. gsx2 is expressed in the early telencephalon and later in the pallium and olfactory bulb. gsx1 and gsx2 are coexpressed in the hypothalamus, preoptic area, and hindbrain, however rarely colocalize in the same cells. gsx1 and gsx2 mutant zebrafish were made with TALENs. gsx1 mutants exhibit stunted growth, however, they survive to adulthood and are fertile. gsx2 mutants experience swim bladder inflation failure that prevents survival. We also observed significantly reduced expression of multiple forebrain patterning distal-less homeobox genes in mutants, and expression of foxp2 was not significantly affected.
Conclusions This work provides novel tools with which other target genes and functions of Gsx1 and Gsx2 can be characterized across the CNS to better understand the unique and overlapping roles of these highly conserved transcription factors. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping