PUBLICATION

Macrophages Rapidly Seal off the Punctured Zebrafish Larval Brain through a Vital Honeycomb Network Structure

Authors
Zou, D., Qin, J., Hu, W., Wei, Z., Zhan, Y., He, Y., Zhao, C., Li, L.
ID
ZDB-PUB-220924-19
Date
2022
Source
International Journal of Molecular Sciences   23(18): (Journal)
Registered Authors
Li, Li, Zhao, CongJian
Keywords
TBI, aggregation, collagen, macrophages, stab
MeSH Terms
  • Animals
  • Brain/metabolism
  • Brain Injuries, Traumatic*/metabolism
  • Collagen/metabolism
  • Edema/metabolism
  • Larva/metabolism
  • Macrophages/metabolism
  • Microglia/metabolism
  • Zebrafish*/metabolism
(all 9)
PubMed
36142462 Full text @ Int. J. Mol. Sci.
Abstract
There is accumulating evidence that macrophages play additional important roles in tissue damage besides their typical phagocytosis. Although the aggregation of macrophages on injured sites has long been observed, few researchers have focused on the role of the overall structure of macrophage aggregation. In this study, we developed a standardized traumatic brain injury (TBI) model in zebrafish larvae to mimic edema and brain tissue spillage symptoms after severe brain trauma. Using time-lapse imaging, we showed that macrophages/microglia in zebrafish larvae responded rapidly and dominated the surface of injured tissue, forming a meaningful honeycomb network structure through their compact aggregation and connection. Disrupting this structure led to fatal edema-like symptoms with severe loss of brain tissue. Using the RNA-Seq, together with the manipulation of in vitro cell lines, we found that collagen IV was indispensable to the formation of honeycomb network structures. Our study thus revealed a novel perspective regarding macrophages forming a protective compact structure with collagen IV. This honeycomb network structure acted as a physical barrier to prevent tissue loss and maintain brain homeostasis after TBI. This study may provide new evidence of macrophages' function for the rapid protection of brain tissue after brain injury.
Genes / Markers
Marker Marker Type Name
cebpaGENECCAAT enhancer binding protein alpha
coro1aGENEcoronin, actin binding protein, 1A
1 - 2 of 2
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Figures
Figure Gallery (8 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cq22TgTransgenic Insertion
    cy22TgTransgenic Insertion
      gl22TgTransgenic Insertion
        hkz04tTgTransgenic Insertion
          hkz011tTgTransgenic Insertion
            knu3TgTransgenic Insertion
              mi2001TgTransgenic Insertion
                zf3773TgTransgenic Insertion
                  zf3774
                    		Insertion
                    1 - 9 of 9
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                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    Target Reagent Reagent Type
                    cebpaCRISPR2-cebpaCRISPR
                    1 - 1 of 1
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                    Fish
                    No data available
                    Antibodies
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    Dendra2EFGDendra2
                    DsRedxEFGDsRedx
                    EGFPEFGEGFP
                    GFPEFGGFP
                    KaedeEFGKaede
                    NTREFGNTR
                    VenusEFGVenus
                    1 - 7 of 7
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                    Mapping
                    No data available
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                    Citation
                    Zou, D., Qin, J., Hu, W., Wei, Z., Zhan, Y., He, Y., Zhao, C., Li, L. (2022) Macrophages Rapidly Seal off the Punctured Zebrafish Larval Brain through a Vital Honeycomb Network Structure. International Journal of Molecular Sciences. 23(18).